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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2010-5-31
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pubmed:abstractText |
In the process of tissue injury and repair, epithelial cells rapidly migrate and form epithelial sheets. Vinexin is a cytoplasmic molecule of the integrin-containing cell adhesion complex localized at focal contacts in vitro. Here, we investigated the roles of vinexin in keratinocyte migration in vitro and wound healing in vivo. Vinexin knockdown using siRNA delayed migration of both HaCaT human keratinocytes and A431 epidermoid carcinoma cells in scratch assay but did not affect cell proliferation. Induction of cell migration by scratching the confluent monolayer culture of these cells activated both EGFR and ERK, and their inhibitors AG1478 and U0126 substantially suppressed scratch-induced keratinocyte migration. Vinexin knockdown in these cells inhibited the scratch-induced activation of EGFR, but not that of ERK, suggesting that vinexin promotes cell migration via activation of EGFR. We further generated vinexin (-/-) mice and isolated their keratinocytes. They similarly showed slow migration in scratch assay. Furthermore, vinexin (-/-) mice exhibited a delay in cutaneous wound healing in both the back skin and tail without affecting the proliferation of keratinocytes. Together, these results strongly suggest a crucial role of vinexin in keratinocyte migration in vitro and cutaneous wound healing in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/SORBS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sh3d4 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1090-2422
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pubmed:author |
pubmed-author:ImaiHiroshiH,
pubmed-author:ItoTakuyaT,
pubmed-author:KiokaNoriyukiN,
pubmed-author:MotoyoshiSohS,
pubmed-author:TakahashiKenzoK,
pubmed-author:UedaKazumitsuK,
pubmed-author:UekawaNatsukoN,
pubmed-author:UmemotoTsutomuT,
pubmed-author:WatanabeHidetoH,
pubmed-author:YamadaMasayasuM,
pubmed-author:YamashitaHiroshiH
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
316
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1728-38
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pubmed:meshHeading |
pubmed-meshheading:20361963-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:20361963-Animals,
pubmed-meshheading:20361963-Base Sequence,
pubmed-meshheading:20361963-Cell Line,
pubmed-meshheading:20361963-Cell Movement,
pubmed-meshheading:20361963-Cell Proliferation,
pubmed-meshheading:20361963-Cells, Cultured,
pubmed-meshheading:20361963-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20361963-Humans,
pubmed-meshheading:20361963-Keratinocytes,
pubmed-meshheading:20361963-Mice,
pubmed-meshheading:20361963-Mice, Inbred C57BL,
pubmed-meshheading:20361963-Mice, Knockout,
pubmed-meshheading:20361963-Muscle Proteins,
pubmed-meshheading:20361963-RNA, Small Interfering,
pubmed-meshheading:20361963-Receptor, Epidermal Growth Factor,
pubmed-meshheading:20361963-Skin,
pubmed-meshheading:20361963-Wound Healing
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pubmed:year |
2010
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pubmed:articleTitle |
Crucial role of vinexin for keratinocyte migration in vitro and epidermal wound healing in vivo.
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pubmed:affiliation |
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan. nkioka@kais.kyoto-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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