Linked Life Data

20360133

Source:http://linkedlifedata.com/resource/pubmed/id/20360133

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-16
pubmed:abstractText
Hyposecretion occurs in most patients early after submandibular gland autotransplantation for severe keratoconjunctivitis sicca. Endogenous transient receptor potential vanilloid subtype 1 (TRPV1) has been recently demonstrated in rabbit submandibular glands, and activation of TRPV1 by capsaicin increases secretion in isolated glands, but the TRPV1-mediated secretory mechanism remains to be elucidated. The purpose of this study was to verify whether activation of TRPV1 by capsaicin could improve the secretion of transplanted gland and its underlying mechanism. The salivary flow of the transplanted glands was significantly decreased, and the mRNA and protein levels of TRPV1 and aquaporin 5 (AQP5) were downregulated in the transplanted glands. Topical capsaicin cream increased secretion and upregulated levels of TRPV1 and AQP5 in transplanted glands. Moreover, in cultured submandibular gland cells, capsaicin increased the mRNA expression of AQP5 and led to redistribution of AQP5 from the cytoplasm to the plasma membrane via TRPV1 activation. Capsaicin enhanced the phosphorylation of extracellular signal-regulated kinase (ERK). Preincubation of cells with PD98059, an inhibitor of ERK kinase, suppressed the capsaicin-induced mRNA expression of AQP5. In summary, the capsaicin-induced secretory mechanism involved activation of TRPV1 and upregulation of AQP5 in an ERK-dependent manner and promoted the redistribution of AQP5 in submandibular gland cells. Activation of TRPV1 may provide a new therapeutic strategy to improve submandibular gland hypofunction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1522-1547
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
299
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G54-62
pubmed:meshHeading
pubmed-meshheading:20360133-Animals, pubmed-meshheading:20360133-Aquaporin 5, pubmed-meshheading:20360133-Capsaicin, pubmed-meshheading:20360133-Cell Membrane, pubmed-meshheading:20360133-Cells, Cultured, pubmed-meshheading:20360133-Cytoplasm, pubmed-meshheading:20360133-Flavonoids, pubmed-meshheading:20360133-Male, pubmed-meshheading:20360133-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:20360133-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:20360133-Phosphorylation, pubmed-meshheading:20360133-Protein Kinase Inhibitors, pubmed-meshheading:20360133-Protein Transport, pubmed-meshheading:20360133-RNA, Messenger, pubmed-meshheading:20360133-Rabbits, pubmed-meshheading:20360133-Regional Blood Flow, pubmed-meshheading:20360133-Saliva, pubmed-meshheading:20360133-Salivation, pubmed-meshheading:20360133-Sensory System Agents, pubmed-meshheading:20360133-Submandibular Gland, pubmed-meshheading:20360133-TRPV Cation Channels, pubmed-meshheading:20360133-Time Factors, pubmed-meshheading:20360133-Transplantation, Autologous, pubmed-meshheading:20360133-Up-Regulation
pubmed:year
2010
pubmed:articleTitle
Activation of transient receptor potential vanilloid subtype 1 increases secretion of the hypofunctional, transplanted submandibular gland.
pubmed:affiliation
Department of Physiology and Pathophysiology, Peking University Health Science Center and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, 100191, P.R. China. pathophy@bjmu.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't