Source:http://linkedlifedata.com/resource/pubmed/id/20357769
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-5-5
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pubmed:databankReference | |
pubmed:abstractText |
Strategies to combat HIV-1 require structural knowledge of envelope proteins from viruses in HIV-1 clade C, the most rapidly spreading subtype in the world. We present a crystal structure containing a clade C gp120 envelope. The structure, a complex between gp120, the host receptor CD4 and the CD4-induced antibody 21c, reveals that the 21c epitope involves contacts with gp120, a nonself antigen, and with CD4, an autoantigen. Binding studies using wild-type and mutant CD4 show that 21c Fab binds CD4 in the absence of gp120, and that binding of 21c to clade C and HIV-2 gp120s requires the crystallographically observed 21c-CD4 interaction. Additional binding data suggest a role for the gp120 V1V2 loop in creating a high-affinity, but slow-forming, epitope for 21c after CD4 binds. These results contribute to a molecular understanding of CD4-induced antibodies and provide the first visualization to our knowledge of a potentially autoreactive antibody Fab complexed with both self and nonself antigens.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1545-9985
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
608-13
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:20357769-Antibodies, Viral,
pubmed-meshheading:20357769-Antigens, CD4,
pubmed-meshheading:20357769-Cell Line,
pubmed-meshheading:20357769-Crystallography, X-Ray,
pubmed-meshheading:20357769-Epitopes,
pubmed-meshheading:20357769-HIV Envelope Protein gp120,
pubmed-meshheading:20357769-HIV Infections,
pubmed-meshheading:20357769-HIV-1,
pubmed-meshheading:20357769-Humans,
pubmed-meshheading:20357769-Models, Molecular
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pubmed:year |
2010
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pubmed:articleTitle |
Structure of a clade C HIV-1 gp120 bound to CD4 and CD4-induced antibody reveals anti-CD4 polyreactivity.
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pubmed:affiliation |
Division of Biology, California Institute of Technology, Pasadena, California, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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