Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-6-23
pubmed:databankReference
pubmed:abstractText
Few studies in the past have examined the genetic diversity and zoonotic potential of Cryptosporidium in dairy cattle in India. To assess the importance of these animals as a source of human Cryptosporidium infections, fecal samples from 180 calves, heifers and adults and 51 farm workers on two dairy farms in West Bengal, India were genotyped by PCR-RFLP analysis of the 18S rRNA gene of Cryptosporidium followed by DNA sequencing of the PCR products. Phylogenetic analysis was carried out on the DNA sequences obtained in the study and those available in GenBank. The overall prevalence of Cryptosporidium in cattle was 11.7% though the infection was more prevalent in younger calves than in adult cattle. The occurrence of Cryptosporidium parvum, Cryptosporidium bovis, Cryptosporidium ryanae and Cryptosporidium andersoni in cattle followed an age-related pattern. A Cryptosporidium suis-like genotype was also detected in a calf. Farm workers were infected with Cryptosporidium hominis, C. parvum and a novel C. bovis genotype. These findings clearly suggest that there is a potential risk of zoonotic transmission of Cryptosporidium infections between cattle and humans on dairy farms in India.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1873-2550
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
171
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-7
pubmed:meshHeading
pubmed-meshheading:20356678-Agriculture, pubmed-meshheading:20356678-Animals, pubmed-meshheading:20356678-Base Sequence, pubmed-meshheading:20356678-Cattle, pubmed-meshheading:20356678-Cattle Diseases, pubmed-meshheading:20356678-Cryptosporidiosis, pubmed-meshheading:20356678-Cryptosporidium, pubmed-meshheading:20356678-DNA, Protozoan, pubmed-meshheading:20356678-Feces, pubmed-meshheading:20356678-Female, pubmed-meshheading:20356678-Humans, pubmed-meshheading:20356678-India, pubmed-meshheading:20356678-Intestinal Diseases, Parasitic, pubmed-meshheading:20356678-Molecular Sequence Data, pubmed-meshheading:20356678-Phylogeny, pubmed-meshheading:20356678-Polymerase Chain Reaction, pubmed-meshheading:20356678-Polymorphism, Restriction Fragment Length, pubmed-meshheading:20356678-RNA, Ribosomal, 18S, pubmed-meshheading:20356678-Sequence Alignment, pubmed-meshheading:20356678-Sequence Analysis, DNA, pubmed-meshheading:20356678-Zoonoses
pubmed:year
2010
pubmed:articleTitle
Molecular characterization and assessment of zoonotic transmission of Cryptosporidium from dairy cattle in West Bengal, India.
pubmed:affiliation
Division of Parasitology, National Institute of Cholera and Enteric Diseases, P-33, C. I. T. Road, Beliaghata, Scheme-XM, Kolkata 700010, West Bengal, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't