Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-4-28
pubmed:abstractText
We evaluated minimal residual disease (MRD) in 91 children with acute lymphoblastic leukemia (ALL) by PCR amplification of clonal rearrangements, immunoglobulin (IgH; VDJ rearrangement, CDR3 region) and T-cell receptor (TCRdelta). Sequential monitoring of MRD was performed at different time points during and after chemotherapy and was correlated to patient outcome. In total, 792 bone marrow samples were assessed for MRD at the end of induction, and during and after treatment completion. MRD positivity at the end of induction was detected in 12% of patients and was associated with high incidence of relapse, 54.55% (p = 0.0002), at 5 years. On the other hand, 88% of patients were MRD-negative at the end of induction and the relapse rate at 5 years was very low, 5%. The frequency of MRD decreased to 16% in the first 6 months of chemotherapy; however, the incidence of relapse in MRD-positive patients remained high, 42.8%. After treatment completion (24-36 months from diagnosis), 32% patients were MRD-positive and the relapse rate was 36.5% (p = 0.0009). Our results indicated that monitoring of MRD constituted an essential prognostic marker, and detection of MRD particularly at the end of induction and after treatment completion was strongly predictive for patient outcome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1029-2403
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
846-52
pubmed:meshHeading
pubmed-meshheading:20350276-Bone Marrow, pubmed-meshheading:20350276-Child, pubmed-meshheading:20350276-Child, Preschool, pubmed-meshheading:20350276-Chromosome Aberrations, pubmed-meshheading:20350276-Cohort Studies, pubmed-meshheading:20350276-DNA, Neoplasm, pubmed-meshheading:20350276-Female, pubmed-meshheading:20350276-Follow-Up Studies, pubmed-meshheading:20350276-Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor, pubmed-meshheading:20350276-Humans, pubmed-meshheading:20350276-In Situ Hybridization, Fluorescence, pubmed-meshheading:20350276-Infant, pubmed-meshheading:20350276-Male, pubmed-meshheading:20350276-Neoplasm, Residual, pubmed-meshheading:20350276-Neoplasm Recurrence, Local, pubmed-meshheading:20350276-Polymerase Chain Reaction, pubmed-meshheading:20350276-Precursor Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:20350276-Prognosis, pubmed-meshheading:20350276-Remission Induction, pubmed-meshheading:20350276-Survival Rate, pubmed-meshheading:20350276-Time Factors
pubmed:year
2010
pubmed:articleTitle
Sequential monitoring of minimal residual disease in acute lymphoblastic leukemia: 7-year experience in a pediatric hematology/oncology unit.
pubmed:affiliation
Hematology/Oncology Unit, First Department of Pediatrics, University of Athens, Aghia Sophia Children's Hospital, Thivon & Levadias, 11527, Goudi-Athens, Greece. katharinakats@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't