Source:http://linkedlifedata.com/resource/pubmed/id/20347917
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-5-17
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pubmed:abstractText |
Bitter melon (Momordica charantia) contains a variety of potentially bioactive compounds which includes two classes of saponins known as cucurbitane and oleanane-type triterpenoids. A bitter melon (BM) extract was investigated for the potential to reduce cell viability, reduce lipid accumulation in differentiating 3T3-L1 cells and affect subsequent adiponectin expression. BM extract contained at least five different triterpenoids and reduced preadipocyte viability with an LC50 concentration after 24h determined to be 0.402+/-0.04 mg/mL, 0.314+/-0.01 mg/mL for 48 h and 0.310+/-0.01 mg/mL for 72 h. BM treatment increased the release of lactate dehydrogenase (LDH) from cells and significantly (p<0.05) increased cells in the G2/M while reducing cells in the G1 phase of the cell cycle. BM treatment of 3T3-L1 cells resulted in a decreased in lipid accumulation and significantly (p<0.05) decreased intracellular triglyceride amount compared to untreated control cells. PPARgamma expression was significantly (p<0.05) down-regulated. Adiponectin expression was significantly (p<0.05) decreased after 12 and 24h of treatment and was increased in response to troglitazone, a positive control. BM extract reduced preadipocyte proliferation by a G2/M arrest of the cell cycle and reduced lipid accumulation of the adipocyte.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adiponectin,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Triterpenes
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1873-6351
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1619-26
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pubmed:meshHeading |
pubmed-meshheading:20347917-3T3-L1 Cells,
pubmed-meshheading:20347917-Adipocytes,
pubmed-meshheading:20347917-Adiponectin,
pubmed-meshheading:20347917-Animals,
pubmed-meshheading:20347917-Cell Cycle,
pubmed-meshheading:20347917-Cell Differentiation,
pubmed-meshheading:20347917-Chromatography, High Pressure Liquid,
pubmed-meshheading:20347917-L-Lactate Dehydrogenase,
pubmed-meshheading:20347917-Mass Spectrometry,
pubmed-meshheading:20347917-Mice,
pubmed-meshheading:20347917-Momordica charantia,
pubmed-meshheading:20347917-PPAR gamma,
pubmed-meshheading:20347917-Plant Extracts,
pubmed-meshheading:20347917-Triterpenes
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pubmed:year |
2010
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pubmed:articleTitle |
Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells.
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pubmed:affiliation |
Department of Chemistry, National University of Singapore, Singapore, Singapore. chmpdg@nus.edu.sg
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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