Linked Life Data

20347780

Source:http://linkedlifedata.com/resource/pubmed/id/20347780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2010-6-4
pubmed:abstractText
This study was designed to determine how the contractility of rat detrusor smooth muscle strips changes in the presence of low concentrations of hydrogen peroxide (H(2)O(2)). The strips were dissected from the base of Sprague-Dawley rat bladders and their contractile responses to a cumulative increase in H(2)O(2) concentration (3 x 10(-6)-3 x 10(-2)g%) were measured. How the duration of exposure to the fixed concentration of 3 x 10(-4)g% H(2)O(2) affected contractility was also examined. Moreover, the effect of 3 x 10(-4)g% H(2)O(2) pretreatment on the response to cumulative increases in the concentrations of phenylephrine or acetylcholine (10(-8)-10(-4)M) was assessed. To elucidate the mechanism by which H(2)O(2) induced contraction, we examined the effect of pretreatment with 10nM Y-27632, 10 microM indomethacin, 10 microM SQ29548, 10 microM verapamil, 10 microM vitamin E, or 1 microM Bay-K 8644 on the contractile responses generated by cumulatively increasing the concentration of H(2)O(2). H(2)O(2)-induced contractile responses in Ca(2+)-free physiological solution were also examined. Low concentrations of H(2)O(2) increased the contractile responses of the strips in a dose-dependent manner but increasing treatment duration decreased these responses. H(2)O(2)-pretreatment significantly augmented the contraction induced by phenylephrine (P<0.05) but had no effect on the response to acetylcholine. Pretreatment with Y-27632, indomethacin, vitamin E, verapamil, and Bay-K 8644 significantly inhibited the H(2)O(2)-induced contraction (P<0.05). SQ 29548-pretreatment had no effect. H(2)O(2) could not increase the contractile responses in Ca(2+)-free physiological solution. Thus, low concentrations of H(2)O(2) may directly affect detrusor smooth muscles and thereby induce detrusor overactivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1879-0712
pubmed:author
pubmed:copyrightInfo
(c) 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
638
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-20
pubmed:meshHeading
pubmed-meshheading:20347780-3-Pyridinecarboxylic acid..., pubmed-meshheading:20347780-Acetylcholine, pubmed-meshheading:20347780-Amides, pubmed-meshheading:20347780-Animals, pubmed-meshheading:20347780-Calcium, pubmed-meshheading:20347780-Dose-Response Relationship, Drug, pubmed-meshheading:20347780-Drug Synergism, pubmed-meshheading:20347780-Hydrogen Peroxide, pubmed-meshheading:20347780-Indomethacin, pubmed-meshheading:20347780-Male, pubmed-meshheading:20347780-Muscle, Smooth, pubmed-meshheading:20347780-Muscle Contraction, pubmed-meshheading:20347780-Phenylephrine, pubmed-meshheading:20347780-Pyridines, pubmed-meshheading:20347780-Rats, pubmed-meshheading:20347780-Rats, Sprague-Dawley, pubmed-meshheading:20347780-Time Factors, pubmed-meshheading:20347780-Urinary Bladder, pubmed-meshheading:20347780-Verapamil, pubmed-meshheading:20347780-Vitamin E
pubmed:year
2010
pubmed:articleTitle
Effect of low concentrations of hydrogen peroxide on the contractile responses of rat detrusor smooth muscle strips.
pubmed:affiliation
Department of Urology, KEPCO Medical Foundation Hanil General Hospital, Seoul, Republic of Korea.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't