Source:http://linkedlifedata.com/resource/pubmed/id/20346366
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2010-5-17
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pubmed:abstractText |
In mouse thymic lymphoma 3SB cells bearing wild type p53, ionizing radiation (IR) and UV light are potent triggers of caspase-3-dependent apoptosis. Although cytochrome c was released from mitochondria as expected, caspase-9 activation was not observed in UV-exposed cells. Laser scanning confocal microscopy analysis showed that caspase-9 is localized in an unusual punctuated pattern in UV-induced apoptotic cells. In agreement with differences in the status of caspase-9 activation between IR and UV, subcellular protein fractionation experiments showed that pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1), normally a part of the apoptosome assembled in response to the release of cytochrome c from mitochondria, and B-cell lymphoma extra long (Bcl-xL), an inhibitor of the change in mitochondrial membrane permeability, were redistributed by the IR-exposure but not by the UV-exposure. Instead of the sequestration of the capase-9/apoptosome activation in UV-induced apoptotic cells, the extrinsic apoptotic signaling generated by caspase-8 activation and consequent activation of B-cell lymphoma extra long (Bid) to release cytochrome c from mitochondria was observed. Thus, the post-mitochondrial apoptotic pathway downstream of cytochrome c release cannot operate the apoptosome function in UV-induced apoptosis in thymic 3SB cells. The intracellular redistribution and sequestration of apoptosis-related proteins upon mitochondrion-based apoptotic signaling was identified as a novel cellular mechanism to respond to DNA damage in an agent type-specific manner. This finding suggests that the kind of the critical ultimate apoptosis-inducing DNA lesion complex form resulting from the agent-specific DNA damage responses is important to determine which of apoptosis signals would be activated.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-5107
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2010 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
688
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
78-87
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pubmed:meshHeading |
pubmed-meshheading:20346366-Animals,
pubmed-meshheading:20346366-Apoptosis,
pubmed-meshheading:20346366-Caspase 3,
pubmed-meshheading:20346366-Caspase 9,
pubmed-meshheading:20346366-Cell Fractionation,
pubmed-meshheading:20346366-Cells, Cultured,
pubmed-meshheading:20346366-Cytochromes c,
pubmed-meshheading:20346366-Enzyme Activation,
pubmed-meshheading:20346366-Mice,
pubmed-meshheading:20346366-Mitochondria,
pubmed-meshheading:20346366-Radiation, Ionizing,
pubmed-meshheading:20346366-Thymus Gland,
pubmed-meshheading:20346366-Ultraviolet Rays
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pubmed:year |
2010
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pubmed:articleTitle |
Differential regulation of caspase-9 by ionizing radiation- and UV-induced apoptotic pathways in thymic cells.
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pubmed:affiliation |
Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shoubara, Hiroshima 727-0023, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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