Linked Life Data

2034234

Source:http://linkedlifedata.com/resource/pubmed/id/2034234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-6-21
pubmed:abstractText
We have studied the effects of a synthetic peptide that is based on the autoinhibitory domain of protein kinase C on tension development in detergent-permeabilized coronary artery smooth muscle. This peptide inhibited two forms of protein kinase C that were isolated from the coronary artery media layer by hydroxylapatite chromatography, with apparent Ki values in the 5-8 microM range. Contractions induced by calcium in the permeabilized arteries were not affected by the peptide (30 microM). Potentiation of calcium-induced contractions by 1 microM phorbol-12,13-dibutyrate was partially inhibited by 10 microM peptide and was completely abolished by 30 microM peptide. These results indicate that phorbol-12,13-dibutyrate potentiates calcium-induced contractions of permeabilized coronary arteries by activation of protein kinase C, but activated protein kinase C is not a requirement for the induction of contractions by calcium alone.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
621-4
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A synthetic pseudosubstrate peptide of protein kinase C inhibits the phorbol-12,13-dibutyrate effect on permeabilized coronary artery smooth muscle.
pubmed:affiliation
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-6600.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.