Source:http://linkedlifedata.com/resource/pubmed/id/20339399
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-5-28
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pubmed:abstractText |
The alarming rise of hospital- and community-associated methicillin-resistant Staphylococcus aureus (HA- and CA-MRSA) infections has prompted a desperate search for novel antibiotics. We discovered the streptogramin etamycin produced by an actinomycete species isolated from the coast of Fiji, the first time this antibiotic has been identified from a marine microbe. Etamycin was extracted and purified from this strain (CNS-575) and identified as a three-rotamer species by 2D NMR spectroscopy. Etamycin demonstrated potent activity against HA- and CA-MRSA in microbroth dilution assays, with minimum inhibitory concentrations (MIC) as low as 1-2 mg l(-1) against HA- and CA-MRSA strains. Furthermore, etamycin was also active against other Gram-positive and several Gram-negative pathogens and was found to be non-cytotoxic at concentrations more than 20-fold above MIC. Etamycin displayed favorable time-kill kinetics compared with the first-line MRSA antibiotic, vancomycin, and also conferred significant protection from mortality in a murine model of systemic lethal MRSA infection. These data emphasize the utility of the marine environment as a relatively untapped source of antibiotics against major drug-resistant human pathogens. These studies will also guide future isolation and preclinical development of depsipeptide anti-MRSA compounds from marine-derived actinomycetes.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/R01 GM084350-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM084350-02,
http://linkedlifedata.com/resource/pubmed/grant/R01GM084350-01,
http://linkedlifedata.com/resource/pubmed/grant/T32 CA121938,
http://linkedlifedata.com/resource/pubmed/grant/T32 GM067550,
http://linkedlifedata.com/resource/pubmed/grant/U01-TW007401-01
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-8820
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
219-24
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pubmed:dateRevised |
2010-12-30
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pubmed:meshHeading |
pubmed-meshheading:20339399-Actinobacteria,
pubmed-meshheading:20339399-Animals,
pubmed-meshheading:20339399-Anti-Bacterial Agents,
pubmed-meshheading:20339399-Female,
pubmed-meshheading:20339399-Macrolides,
pubmed-meshheading:20339399-Magnetic Resonance Spectroscopy,
pubmed-meshheading:20339399-Methicillin-Resistant Staphylococcus aureus,
pubmed-meshheading:20339399-Mice,
pubmed-meshheading:20339399-Microbial Sensitivity Tests,
pubmed-meshheading:20339399-Staphylococcal Infections
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pubmed:year |
2010
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pubmed:articleTitle |
Activity of the streptogramin antibiotic etamycin against methicillin-resistant Staphylococcus aureus.
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pubmed:affiliation |
Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California-San Diego, La Jolla, CA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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