Linked Life Data

20337706

Source:http://linkedlifedata.com/resource/pubmed/id/20337706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-5-28
pubmed:abstractText
Stable isotope probing of prokaryotic DNA was used to determine active prokaryotes using (13)C-labelled substrates (glucose, acetate, CO(2)) in sediment slurries from different biogeochemical zones of the Severn Estuary, UK. Multiple, low concentrations (5 x 100 microM) of (13)C-substrate additions and short-term incubations (7 days) were used to minimize changes in the prokaryotic community, while achieving significant (13)C-incorporation. Analysis demonstrated clear metabolic activity within all slurries, although neither the net sulphate removal nor CH(4) production occurred in the anaerobic sulphate reduction and methanogenesis zone slurries. Some similarities occurred in the prokaryotic populations that developed in different sediment slurries, particularly in the aerobic and dysaerobic zone slurries with (13)C-glucose, which were dominated by Gammaproteobacteria and Marine Group 1 Archaea, whereas both anaerobic sediment slurries incubated with (13)C-acetate showed incorporation into Epsilonproteobacteria and other bacteria, with the sulphate reduction zone slurry also showing (13)C-acetate utilization by Miscellaneous Crenarchaeotic Group Archaea. The lower potential energy methanogenesis zone slurries were the only conditions where no (13)C-incorporation into Archaea occurred, despite Bacteria being labelled; this was surprising because Archaea have been suggested to be adapted to low-energy conditions. Overall, our results highlight that uncultured prokaryotes play important ecological roles in tidal sediments of the Severn Estuary, providing new metabolic information for novel groups of Archaea and suggesting broader metabolisms for largely uncultivated Bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1574-6941
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-97
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Prokaryotic functional diversity in different biogeochemical depth zones in tidal sediments of the Severn Estuary, UK, revealed by stable-isotope probing.
pubmed:affiliation
Cardiff School of Biosciences, Cardiff University, Wales, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't