Linked Life Data

20336493

Source:http://linkedlifedata.com/resource/pubmed/id/20336493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-2-25
pubmed:abstractText
This study investigated the effects of sodium selenite (Se) and of vitamin E (D-?-tochopherol) on the deposition of type I collagen by human LX-2 stellate cells. The cultured cells were treated with or without Se or vitamin E and with or without transforming growth factor ?1 (TGF?1). The combination of Se and vitamin E, but not either alone, protected against hepatic fibrosis by decreasing TGF?1-mediated collagen secretion and accumulation by the stellate cells. This protective effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen. Effects of Se and vitamin E in decreasing ?(1)(I) collagen mRNA and increasing apoptosis of stellate cells indicate decreased formation of collagen, while decreases in transglutaminase 2, which catalyze cross-linking of collagen, lead to decreased stability of the secreted collagen. Effects of Se and vitamin E on reducing tissue inhibitor metalloproteinase 1 (TIMP-1) are associated with increased degradation. The combination of Se and vitamin E decreased lipid peroxidation, while Se alone increased the activity of the antioxidant enzyme thioredoxin reductase. In conclusion, the combination of Se and vitamin E protected against TGF?1-mediated hepatic fibrosis by decreasing TGF?1-mediated type I collagen accumulation by stellate cells. This effect is due to a combination of decreased formation, decreased stability and increased degradation of the collagen.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1559-0720
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
82-94
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
The combination of selenium and vitamin E inhibits type I collagen formation in cultured hepatic stellate cells.
pubmed:affiliation
Department of Medicine, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205-2195, USA. emezey@jhmi.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.