Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-6-26
|
| pubmed:abstractText |
A stabilized hybridoma cell line secreting anti-retinoic acid monoclonal antibodies of subclass IgG1 with kappa chains was produced by fusing NS-1 myeloma cells with the spleen cells from BALB/c female mice immunized with all-trans-4-oxoretinoic acid-oxime-chicken IgG conjugate. The antibody titer of mice ascitic fluid ranged from 1/12,800 to 1/25,600, as determined by competitive indirect enzyme-linked immunosorbent assay (ELISA). 50% inhibition dosage of all-trans-retinoic acid at a 1/20,000 dilution of mice ascitic fluid was 6.6 ng/ml, as determined by ELISA. The anti-retinoic acid monoclonal antibody was generated in mice ascitic fluid and purified by protein G affinity chromatography. Cross-reactivity of the monoclonal antibody was determined at 0.1 microgram/ml concentration of retinoids and indicated high specificity to both all-trans-retinoic acid (86% inhibition) and 13-cis-retinoic acid (87% inhibition), and strong cross-reactivity with 4-oxoretinoic acid (77%) and 4-oxoretinoic acid oxime (109%). Specificity was confirmed by the horseradish peroxidase-linked immunostaining method and immunoradioassay. The affinity constant of the monoclonal antibody, K, was determined to be 3.6 X 10(9) l/mol. A calibration curve for retinoic acid using the monoclonal antibody to retinoic acid was developed; the detection limit for all-trans-retinoic acid is 1 ng/ml in the competitive indirect ELISA. The antibody counteracts the effect of retinoic acid on growth inhibition and differentiation in HL-60 cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
138
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
211-23
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2033274-Animals,
pubmed-meshheading:2033274-Antibodies, Monoclonal,
pubmed-meshheading:2033274-Antibody Specificity,
pubmed-meshheading:2033274-Binding, Competitive,
pubmed-meshheading:2033274-Cell Line,
pubmed-meshheading:2033274-Cross Reactions,
pubmed-meshheading:2033274-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2033274-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2033274-Female,
pubmed-meshheading:2033274-Hybridomas,
pubmed-meshheading:2033274-Immunoenzyme Techniques,
pubmed-meshheading:2033274-Immunoglobulin G,
pubmed-meshheading:2033274-Mice,
pubmed-meshheading:2033274-Mice, Inbred BALB C,
pubmed-meshheading:2033274-Tretinoin
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Production of a hybridoma cell line secreting retinoic acid-specific monoclonal antibody.
|
| pubmed:affiliation |
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824-1224.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|