Linked Life Data

20331531

Source:http://linkedlifedata.com/resource/pubmed/id/20331531

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-7-14
pubmed:abstractText
The common neurodegenerative disorder known as Alzheimer's disease is characterized by cerebral neuritic plaques of amyloid beta (Abeta) peptide. Plaque formation is related to the highly aggregative property of this peptide, because it polymerizes to form insoluble plaques or fibrils causing neurotoxicity. Here, we expressed Abeta peptide as a new causing agent to endoplasmic reticulum (ER) stress to study ER stress occurred in plant. When the dimer of Abeta(1-42) peptide was expressed in maturing seed under the control of the 2.3-kb glutelin GluB-1 promoter containing its signal peptide, a maximum of about 8 mug peptide per grain accumulated and was deposited at the periphery of distorted ER-derived PB-I protein bodies. Synthesis of Abeta peptide in the ER lumen severely inhibited the synthesis and deposition of seed storage proteins, resulting in the generation of many small and abnormally appearing PB bodies. This ultrastructural change was accounted for by ER stress leading to the accumulation of aggregated Abeta peptide in the ER lumen and a coordinated increase in ER-resident molecular chaperones such as BiPs and PDIs in Abeta-expressing plants. Microarray analysis also confirmed that expression of several BiPs, PDIs and OsbZIP60 containing putative transmembrane domains was affected by the ER stress response. Abeta-expressing transgenic rice kernels exhibited an opaque and shrunken phenotype. When grain phenotype and expression levels were compared among transgenic rice grains expressing several different recombinant peptides, such detrimental effects on grain phenotype were correlated with the expressed peptide causing ER stress rather than expression levels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1467-7652
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
691-718
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Analysis of ER stress in developing rice endosperm accumulating beta-amyloid peptide.
pubmed:affiliation
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't