Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
15
|
| pubmed:dateCreated |
1991-6-25
|
| pubmed:abstractText |
The apoC-III promoter region -86 to -74 is recognized by two nuclear factors designated CIIIB1 and CIIIB2 (NF-BA1) which are both activators of apoC-III gene transcription (Ogami, K., Hadzopoulou-Cladaras, M., Cladaras, C., and Zannis, V. I. (1990) J. Biol. Chem. 265, 9808-9815). In this communication we report the purification of factor CIIIB1 from rat liver nuclear extracts. The purification procedure included anion- and cation-exchange chromatography, DNA sequence-specific affinity chromatography, and heat treatment at 85 degrees C for 5 min. The ligand used for affinity chromatography was a mutated apoC-III -90 to -73 promoter sequence which binds only the CIIIB1 factor. The purified protein was identified as a poly-peptide of Mr 41,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and photoaffinity cross-linking. The binding site of CIIIB1, defined by DNase I footprinting and methylation interference assays, contains the octameric motif CAGGTGAC. Nucleotide substitutions within this sequence abolished the binding of the purified factor. DNase I footprinting analysis showed that purified CIIIB1 protein protects the apoA-II promoter region -65 to -48 which contains an identical octameric CAGGTGAC motif in the antisense strand suggesting that CIIIB1 may also play a role in apoA-II gene regulation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein C-III,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins C,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
266
|
| pubmed:geneSymbol |
apoC-III
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9640-6
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2033057-Animals,
pubmed-meshheading:2033057-Apolipoprotein C-III,
pubmed-meshheading:2033057-Apolipoproteins C,
pubmed-meshheading:2033057-Base Sequence,
pubmed-meshheading:2033057-Binding Sites,
pubmed-meshheading:2033057-Chromatography, Liquid,
pubmed-meshheading:2033057-Cross-Linking Reagents,
pubmed-meshheading:2033057-DNA Fingerprinting,
pubmed-meshheading:2033057-DNA-Binding Proteins,
pubmed-meshheading:2033057-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2033057-Gene Expression Regulation,
pubmed-meshheading:2033057-Humans,
pubmed-meshheading:2033057-Liver,
pubmed-meshheading:2033057-Molecular Sequence Data,
pubmed-meshheading:2033057-Nuclear Proteins,
pubmed-meshheading:2033057-Promoter Regions, Genetic,
pubmed-meshheading:2033057-Rats,
pubmed-meshheading:2033057-Trans-Activators
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Purification and characterization of a heat stable nuclear factor CIIIB1 involved in the regulation of the human ApoC-III gene.
|
| pubmed:affiliation |
Department of Medicine Housman Medical Research Center, Boston University, Medical Center, Massachusetts 02118.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|