Source:http://linkedlifedata.com/resource/pubmed/id/20309538
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-6-3
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| pubmed:abstractText |
Vibrio coralliilyticus is a global marine pathogen that has been found to cause disease in several marine organisms, including corals. This study is the first report of the isolation of V. coralliilyticus from a diseased Caribbean octocoral, Pseudopterogorgia americana. Five sister phylotypes were positively identified using 16S rRNA gene sequencing, recA probes specific for V. coralliilyticus, and rep-PCR fingerprinting. The antimicrobial resistance was compared between pathogenic strains of V. coralliilyticus and the Caribbean strains. First, the antimicrobial resistance of V. coralliilyticus-type strain ATCC BAA-450 was determined using an agar-overlay antimicrobial bioassay at 24 degrees C and 27 degrees C, temperatures which are relevant to its known temperature-dependent virulence. From 108 distinct bacteria isolated from P. americana, 12 inhibited the V. coralliilyticus-type strain at 24 degrees C and five at 27 degrees C. Next, the phenotypic comparison of two Caribbean phylotypes and three V. coralliilyticus reference strains against a subset of 30 bacteria demonstrated a similar resistance trend. At both temperatures, the reference strains were inhibited by three bacteria isolates, while the Caribbean strains were inhibited by four to nine bacteria. Additionally, V. coralliilyticus-type strain ATCC BAA-450 and one of the Caribbean strains were inhibited by a higher number of bacteria at 24 degrees C compared with 27 degrees C. Together, these results highlight that V. coralliilyticus strains have antimicrobial resistance to the majority of coral-associated bacteria tested, which may be temperature-dependent in some strains. Furthermore, all V. coralliilyticus strains tested showed multi-drug resistance to a range of 11-16 (out of 26) commercial antibiotics. This study establishes V. coralliilyticus in association with a Caribbean octocoral and demonstrates its resistance to the antimicrobial activity of coral-associated bacteria and to commercial antibiotics.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1432-184X
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| pubmed:author |
pubmed-author:JohnsonWesley RWR,
pubmed-author:KimesNikole ENE,
pubmed-author:MoellerPeter D RPD,
pubmed-author:MorrisPamela JPJ,
pubmed-author:NelsonKaren EKE,
pubmed-author:SmithGarriet WGW,
pubmed-author:TorralbaManolitoM,
pubmed-author:VizcainoMaria IMI,
pubmed-author:WeilErnestoE,
pubmed-author:WilliamsKatherineK
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| pubmed:issnType |
Electronic
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| pubmed:volume |
59
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
646-57
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| pubmed:meshHeading |
pubmed-meshheading:20309538-Animals,
pubmed-meshheading:20309538-Anthozoa,
pubmed-meshheading:20309538-Anti-Bacterial Agents,
pubmed-meshheading:20309538-Caribbean Region,
pubmed-meshheading:20309538-DNA, Bacterial,
pubmed-meshheading:20309538-DNA Fingerprinting,
pubmed-meshheading:20309538-Drug Resistance, Bacterial,
pubmed-meshheading:20309538-Microbial Sensitivity Tests,
pubmed-meshheading:20309538-Phylogeny,
pubmed-meshheading:20309538-RNA, Ribosomal, 16S,
pubmed-meshheading:20309538-Vibrio
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| pubmed:year |
2010
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| pubmed:articleTitle |
Antimicrobial resistance of the coral pathogen Vibrio coralliilyticus and Caribbean sister phylotypes isolated from a diseased octocoral.
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| pubmed:affiliation |
Molecular and Cellular Biology and Pathobiology, Medical University of South Carolina, Charleston, SC 29412, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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