Source:http://linkedlifedata.com/resource/pubmed/id/20308383
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2010-5-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
A carbapenem-resistant Pseudomonas stutzeri strain isolated from a Dutch patient was analyzed in detail. This isolate produced a metallo-beta-lactamase (MBL) whose gene, with 43.5% GC content, was cloned and expressed in Escherichia coli. beta-Lactamase DIM-1 (for Dutch imipenemase) was weakly related to other Ambler class B beta-lactamases, sharing <52% amino acid identity with the most closely related MBL, GIM-1, and 45% identity with IMP-type MBLs. The beta-Lactamase DIM-1 significantly hydrolyzed broad-spectrum cephalosporins and carbapenems and spared aztreonam. This MBL gene was embedded in a class 1 integron containing two other gene cassettes, encoding resistance to aminoglycosides and disinfectants, that was located on a 70-kb plasmid.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
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| pubmed:issn |
1098-6596
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
54
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2420-4
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| pubmed:dateRevised |
2011-3-3
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| pubmed:meshHeading |
pubmed-meshheading:20308383-Amino Acid Sequence,
pubmed-meshheading:20308383-Bacterial Proteins,
pubmed-meshheading:20308383-Base Sequence,
pubmed-meshheading:20308383-Cloning, Molecular,
pubmed-meshheading:20308383-DNA, Bacterial,
pubmed-meshheading:20308383-Drug Resistance, Bacterial,
pubmed-meshheading:20308383-Genes, Bacterial,
pubmed-meshheading:20308383-Humans,
pubmed-meshheading:20308383-Integrons,
pubmed-meshheading:20308383-Middle Aged,
pubmed-meshheading:20308383-Molecular Sequence Data,
pubmed-meshheading:20308383-Netherlands,
pubmed-meshheading:20308383-Pseudomonas Infections,
pubmed-meshheading:20308383-Pseudomonas stutzeri,
pubmed-meshheading:20308383-Sequence Homology, Amino Acid,
pubmed-meshheading:20308383-Substrate Specificity,
pubmed-meshheading:20308383-beta-Lactamases
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Characterization of DIM-1, an integron-encoded metallo-beta-lactamase from a Pseudomonas stutzeri clinical isolate in the Netherlands.
|
| pubmed:affiliation |
Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|