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rdf:type |
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lifeskim:mentions |
umls-concept:C0001516,
umls-concept:C0003320,
umls-concept:C0004573,
umls-concept:C0017262,
umls-concept:C0026544,
umls-concept:C0205341,
umls-concept:C0430991,
umls-concept:C0740302,
umls-concept:C1166650,
umls-concept:C1171362,
umls-concept:C1257994,
umls-concept:C1366516,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1549781,
umls-concept:C1706474,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2603363
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|
pubmed:issue |
2
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pubmed:dateCreated |
2010-5-4
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pubmed:abstractText |
A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1873-0329
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
294-7
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pubmed:meshHeading |
pubmed-meshheading:20304092-Amino Acid Sequence,
pubmed-meshheading:20304092-Animals,
pubmed-meshheading:20304092-Antibodies, Protozoan,
pubmed-meshheading:20304092-Antigens, Protozoan,
pubmed-meshheading:20304092-Babesia bovis,
pubmed-meshheading:20304092-Babesiosis,
pubmed-meshheading:20304092-Cattle,
pubmed-meshheading:20304092-Cattle Diseases,
pubmed-meshheading:20304092-Cell Adhesion Molecules,
pubmed-meshheading:20304092-Erythrocytes,
pubmed-meshheading:20304092-Molecular Sequence Data,
pubmed-meshheading:20304092-N-Acetylneuraminic Acid,
pubmed-meshheading:20304092-Neutralization Tests,
pubmed-meshheading:20304092-Organelles,
pubmed-meshheading:20304092-Protozoan Proteins,
pubmed-meshheading:20304092-Recombinant Proteins,
pubmed-meshheading:20304092-Sequence Alignment
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pubmed:year |
2010
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pubmed:articleTitle |
Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain.
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pubmed:affiliation |
Biomolecular Diagnostic Laboratory, ITQB/IBET, Universidade Nova de Lisboa, Av. da Republica, Apt 12, Oeiras, Portugal. msilva@itqb.unl.pt
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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