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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-5-24
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pubmed:abstractText |
A rapid and comprehensive protocol, which combines simple purification and liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS), was developed for identification of arboviruses in infected culture fluid. Using this protocol, various arboviruses were detected including uncommon viruses that were described previously as Banna virus and Yunnan orbivirus. This approach is useful for the rapid screening of viral samples that cannot be identified by conventional gene amplification or immunological methods.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1879-0984
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pubmed:author |
pubmed-author:EndoYushirouY,
pubmed-author:GuillermoPosadas HPH,
pubmed-author:HasebeFutoshiF,
pubmed-author:InoueShingoS,
pubmed-author:LoanDo Phuongdo P,
pubmed-author:MoritaKouichiK,
pubmed-author:NabeshimaTakeshiT,
pubmed-author:NatividadFilipinas FFF,
pubmed-author:NgaPhan ThiPT,
pubmed-author:OkamotoKentaK,
pubmed-author:TrangBui MinhBM,
pubmed-author:YuFuxunF
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pubmed:copyrightInfo |
Copyright 2010 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-6
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pubmed:meshHeading |
pubmed-meshheading:20304016-Aedes,
pubmed-meshheading:20304016-Animals,
pubmed-meshheading:20304016-Arboviruses,
pubmed-meshheading:20304016-Cell Line,
pubmed-meshheading:20304016-Cercopithecus aethiops,
pubmed-meshheading:20304016-Chromatography, Liquid,
pubmed-meshheading:20304016-Proteome,
pubmed-meshheading:20304016-Proteomics,
pubmed-meshheading:20304016-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:20304016-Viral Proteins,
pubmed-meshheading:20304016-Virology,
pubmed-meshheading:20304016-Virus Cultivation
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pubmed:year |
2010
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pubmed:articleTitle |
Development of a rapid and comprehensive proteomics-based arboviruses detection system.
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pubmed:affiliation |
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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