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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2010-3-22
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pubmed:abstractText |
Polymerization of actin filaments is the primary source of motility in lamellipodia and it is controlled by a variety of regulatory proteins. The underlying molecular mechanisms are only partially understood and a precise determination of dynamical properties of force generation is necessary. Using optical tweezers, we have measured with millisecond (ms) temporal resolution and picoNewton (pN) sensitivity the force-velocity (Fv) relationship and the power dissipated by lamellipodia of dorsal root ganglia neurons. When force and velocity are averaged over 3-5 s, the Fv relationships can be flat. On a finer timescale, random occurrence of fast growth and subsecond retractions become predominant. The maximal power dissipated by lamellipodia over a silica bead with a diameter of 1 microm is 10(-16) W. Our results clarify the dynamical properties of force generation: i), force generation is a probabilistic process; ii), underlying biological events have a bandwidth up to at least 10 Hz; and iii), fast growth of lamellipodia leading edge alternates with local retractions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20303855-10629223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20303855-10934315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20303855-11231595,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1542-0086
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
17
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
979-88
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pubmed:dateRevised |
2011-7-26
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pubmed:meshHeading |
pubmed-meshheading:20303855-Animals,
pubmed-meshheading:20303855-Cell Movement,
pubmed-meshheading:20303855-Cells, Cultured,
pubmed-meshheading:20303855-Computer Simulation,
pubmed-meshheading:20303855-Models, Biological,
pubmed-meshheading:20303855-Models, Chemical,
pubmed-meshheading:20303855-Models, Statistical,
pubmed-meshheading:20303855-Motion,
pubmed-meshheading:20303855-Neurons,
pubmed-meshheading:20303855-Pseudopodia,
pubmed-meshheading:20303855-Rats,
pubmed-meshheading:20303855-Rats, Wistar,
pubmed-meshheading:20303855-Stress, Mechanical
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pubmed:year |
2010
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pubmed:articleTitle |
Force generation in lamellipodia is a probabilistic process with fast growth and retraction events.
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pubmed:affiliation |
International School for Advanced Studies (SISSA-ISAS), Trieste 34149, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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