Source:http://linkedlifedata.com/resource/pubmed/id/20303598
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2010-5-7
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| pubmed:abstractText |
In this paper we explore the use of fluorescently labeled cytochrome c peroxidase (CcP) from baker's yeast for monitoring nitric oxide (NO) down to the sub-micromolar level, by means of a FRET (Förster Resonance Energy Transfer) mechanism. The binding affinity constant (K(d)) for the NO binding to CcP was determined to be 10+/-1.5 microM. The rate of NO dissociation from the CcP (k(off)) and the second order rate constant for the NO association (k(on)) were found to be 0.22+/-0.08 min(-1) and 0.024+/-0.002 microM(-1) min(-1) respectively. The immobilization of fluorescently labeled CcP into a polymeric matrix for use in a solid state NO sensing device was also explored. The results provide proof-of-principle that labeled CcP can be successfully implemented in a fast, simple, quantitative and sensitive NO sensing device.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
1873-3344
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
104
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
619-24
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| pubmed:meshHeading |
pubmed-meshheading:20303598-Biosensing Techniques,
pubmed-meshheading:20303598-Chromatography, High Pressure Liquid,
pubmed-meshheading:20303598-Fluorescence Resonance Energy Transfer,
pubmed-meshheading:20303598-Molecular Structure,
pubmed-meshheading:20303598-Nitric Oxide,
pubmed-meshheading:20303598-Spectrometry, Mass, Electrospray Ionization
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| pubmed:year |
2010
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| pubmed:articleTitle |
A FRET-based biosensor for NO detection.
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| pubmed:affiliation |
Dipartimento di Chimica, Università di Salerno, via Ponte Don Melillo, Fisciano (Sa), I84084 Italy.
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| pubmed:publicationType |
Journal Article
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