| pubmed-article:2026686 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2026686 | lifeskim:mentions | umls-concept:C0872318 | lld:lifeskim |
| pubmed-article:2026686 | lifeskim:mentions | umls-concept:C0597178 | lld:lifeskim |
| pubmed-article:2026686 | lifeskim:mentions | umls-concept:C0348000 | lld:lifeskim |
| pubmed-article:2026686 | lifeskim:mentions | umls-concept:C1708237 | lld:lifeskim |
| pubmed-article:2026686 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:2026686 | pubmed:dateCreated | 1991-6-10 | lld:pubmed |
| pubmed-article:2026686 | pubmed:abstractText | A commercially available particle beam interface developed by the Vestec Corporation has been coupled to a double-focusing high-resolution mass spectrometer (VG ZAB 2F) via a momentum separator. An ultraviolet detector was in-line with the interface and allowed monitoring of the eluting materials. With this instrumentation, complete electron-impact (EI) mass spectra on synthetic mixtures of steroids, nucleosides, and several derivatives of amino acids were recorded. Amongst these the EI mass spectra of 2,4-dinitrophenyl, 9-fluorenylmethoxycarbonyl and phenylthiohydantoin derivatives were obtained. The standard LC conditions used here (250 mm x 4.6 mm I.D., reversed-phase C18 column, 1.0 or 1.5 ml/min flow-rate, isocratic or gradient programming) allowed separation of the mixtures. The resulting mass spectra were compared with those obtained using a direct insertion probe. The agreement was excellent in most cases. Sensitivity measurements were performed on cholesterol and caffeine. A complete low-resolution mass spectrum can be obtained on 100 ng of cholesterol. Single-ion monitoring of the M+.of 200 pg of caffeine gave a 4:1 signal-to-noise ratio at m/z 194. Complete high-resolution mass spectra were obtained on 5 micrograms of cholesterol (loop injection) and on every peak of a five-steroid mixture of 5 micrograms each. The accuracy of the mass measurements were better than 5 m.m.u. for most cases. Three to four scans could be obtained for each liquid chromatographic peak. Mass-analyzed ion kinetic energy spectra were recorded on the M+.of 2.5 micrograms of cholesterol at m/z 386. Similarly the B/E linked scan of the same ion was recorded on 2.5 micrograms and 500 ng. | lld:pubmed |
| pubmed-article:2026686 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2026686 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2026686 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2026686 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2026686 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:2026686 | pubmed:author | pubmed-author:BaczynskyjLL | lld:pubmed |
| pubmed-article:2026686 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2026686 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:2026686 | pubmed:volume | 562 | lld:pubmed |
| pubmed-article:2026686 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2026686 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2026686 | pubmed:pagination | 13-29 | lld:pubmed |
| pubmed-article:2026686 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2026686 | pubmed:meshHeading | pubmed-meshheading:2026686-... | lld:pubmed |
| pubmed-article:2026686 | pubmed:meshHeading | pubmed-meshheading:2026686-... | lld:pubmed |
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| pubmed-article:2026686 | pubmed:meshHeading | pubmed-meshheading:2026686-... | lld:pubmed |
| pubmed-article:2026686 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2026686 | pubmed:articleTitle | Particle beam liquid chromatography-mass spectrometry on a double-focusing sector instrument. | lld:pubmed |
| pubmed-article:2026686 | pubmed:affiliation | Physical and Analytical Chemistry Research, Upjohn Company, Kalamazoo, MI 49001. | lld:pubmed |
| pubmed-article:2026686 | pubmed:publicationType | Journal Article | lld:pubmed |
