Linked Life Data

2026686

Source:http://linkedlifedata.com/resource/pubmed/id/2026686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1991-6-10
pubmed:abstractText
A commercially available particle beam interface developed by the Vestec Corporation has been coupled to a double-focusing high-resolution mass spectrometer (VG ZAB 2F) via a momentum separator. An ultraviolet detector was in-line with the interface and allowed monitoring of the eluting materials. With this instrumentation, complete electron-impact (EI) mass spectra on synthetic mixtures of steroids, nucleosides, and several derivatives of amino acids were recorded. Amongst these the EI mass spectra of 2,4-dinitrophenyl, 9-fluorenylmethoxycarbonyl and phenylthiohydantoin derivatives were obtained. The standard LC conditions used here (250 mm x 4.6 mm I.D., reversed-phase C18 column, 1.0 or 1.5 ml/min flow-rate, isocratic or gradient programming) allowed separation of the mixtures. The resulting mass spectra were compared with those obtained using a direct insertion probe. The agreement was excellent in most cases. Sensitivity measurements were performed on cholesterol and caffeine. A complete low-resolution mass spectrum can be obtained on 100 ng of cholesterol. Single-ion monitoring of the M+.of 200 pg of caffeine gave a 4:1 signal-to-noise ratio at m/z 194. Complete high-resolution mass spectra were obtained on 5 micrograms of cholesterol (loop injection) and on every peak of a five-steroid mixture of 5 micrograms each. The accuracy of the mass measurements were better than 5 m.m.u. for most cases. Three to four scans could be obtained for each liquid chromatographic peak. Mass-analyzed ion kinetic energy spectra were recorded on the M+.of 2.5 micrograms of cholesterol at m/z 386. Similarly the B/E linked scan of the same ion was recorded on 2.5 micrograms and 500 ng.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
562
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-29
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Particle beam liquid chromatography-mass spectrometry on a double-focusing sector instrument.
pubmed:affiliation
Physical and Analytical Chemistry Research, Upjohn Company, Kalamazoo, MI 49001.
pubmed:publicationType
Journal Article