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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-6-12
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pubmed:abstractText |
A rapid purification method is reported for bovine brain neurogranin, a calmodulin-binding protein kinase C (PKC) substrate. This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium: Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2026258-Amino Acid Sequence,
pubmed-meshheading:2026258-Animals,
pubmed-meshheading:2026258-Brain,
pubmed-meshheading:2026258-Calmodulin,
pubmed-meshheading:2026258-Calmodulin-Binding Proteins,
pubmed-meshheading:2026258-Cattle,
pubmed-meshheading:2026258-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2026258-Molecular Sequence Data,
pubmed-meshheading:2026258-Nerve Tissue Proteins,
pubmed-meshheading:2026258-Neurogranin,
pubmed-meshheading:2026258-Phosphorylation,
pubmed-meshheading:2026258-Protein Kinase C,
pubmed-meshheading:2026258-Solubility
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pubmed:year |
1991
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pubmed:articleTitle |
A rapid purification method for neurogranin, a brain specific calmodulin-binding protein kinase C substrate.
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pubmed:affiliation |
Laboratoire de Neurobiologie Ontogénique, Centre de Neurochimie du CNRS, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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