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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-6-11
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pubmed:abstractText |
Autoantibodies against microsomal antigen of liver and kidney (LKM-1) are diagnostic markers for a subgroup of autoimmune chronic active hepatitis (AI-CAH). Cytochrome P450dbl, now classified as cytochrome P450 IID6, is the major antigen of LKM-1 antibodies. Immunohistological studies suggest that hepatic injury in AI-CAH is mediated by liver-infiltrating T cells. In the present study the specificity and function of liver-infiltrating T cells was analysed at the clonal level. Phenotypical characterization of 189 T cell clones isolated from four liver biopsies of LKM-1 antibody-positive patients showed an enrichment of CD4+ CD8- T cell clones proliferated specifically in the presence of recombinant human LKM-1 antigen (rLKM). This reaction was restricted to autologous antigen-presenting cells and to HLA class II molecules. In order to see whether rLKM was also recognized by peripheral blood T lymphocytes (PBL) we tested the proliferative response of PBL from several individuals. PBL from three of the four patients with LKM-1 antibody-positive AI-CAH proliferated to rLKM, whereas no response was seen with PBL from patients with LKM-1 antibody-negative chronic liver diseases and from healthy blood donors. These data demonstrate that the LKM-1 antigen is recognized by liver-infiltrating T cells in LKM-1 antibody-positive AI-CAH. For further functional characterization, liver-derived T cell clones were tested for their cytotoxic activity. In the presence of phytohaemagglutinin 24 out of 26 CD4- CD8+ but also 20 out of 63 CD4+ CD8- T cell clones lysed autologous as well as allogenic EBV-transformed B cell lines or K562 cells. Five CD4- CD8+ T cell clones lysed autologous but not allogenic B cell lines spontaneously in a HLA class I-restricted manner. Although the antigen specificity of these clones is still unknown the data show the presence of autoreactive T cells at the site of inflammation that could contribute in the pathogenesis of AI-CAH.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2391070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2420472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2466049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2477450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2503564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2572541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2681396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-2957133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-3123997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-3186722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-3308211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-3679093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-4587503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-6187843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-6307807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-6406607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-6467682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2025956-71400
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
297-302
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2025956-Adolescent,
pubmed-meshheading:2025956-Adult,
pubmed-meshheading:2025956-Antigens, CD,
pubmed-meshheading:2025956-Autoantibodies,
pubmed-meshheading:2025956-Autoimmune Diseases,
pubmed-meshheading:2025956-Clone Cells,
pubmed-meshheading:2025956-Cytotoxicity, Immunologic,
pubmed-meshheading:2025956-Female,
pubmed-meshheading:2025956-Hepatitis, Chronic,
pubmed-meshheading:2025956-Humans,
pubmed-meshheading:2025956-Immunophenotyping,
pubmed-meshheading:2025956-Liver,
pubmed-meshheading:2025956-Lymphocyte Activation,
pubmed-meshheading:2025956-Male,
pubmed-meshheading:2025956-Recombinant Proteins,
pubmed-meshheading:2025956-T-Lymphocytes,
pubmed-meshheading:2025956-Thymidine
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pubmed:year |
1991
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pubmed:articleTitle |
Clonal analysis of liver-infiltrating T cells in patients with LKM-1 antibody-positive autoimmune chronic active hepatitis.
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pubmed:affiliation |
First Department of Medicine, University of Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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