| pubmed-article:2024702 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2024702 | lifeskim:mentions | umls-concept:C0440744 | lld:lifeskim |
| pubmed-article:2024702 | lifeskim:mentions | umls-concept:C0022686 | lld:lifeskim |
| pubmed-article:2024702 | lifeskim:mentions | umls-concept:C1441616 | lld:lifeskim |
| pubmed-article:2024702 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
| pubmed-article:2024702 | lifeskim:mentions | umls-concept:C1977560 | lld:lifeskim |
| pubmed-article:2024702 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2024702 | pubmed:dateCreated | 1991-6-4 | lld:pubmed |
| pubmed-article:2024702 | pubmed:abstractText | Human granzyme B antigen is expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme B was generated using a prokaryotic expression vector under the control of T7 transcription and translation signals. The 25-kd recombinant protein (granzyme B) was used to develop a rabbit polyclonal antiserum. Purified anti-granzyme B antibodies were used to detect the antigen expression in cytotoxic cells in human tissues. Using the avidin-biotin-complex peroxidase technique, formalin-fixed, paraffin-embedded tissue sections from patients with acute mild or moderate allograft cardiac rejection were stained. A constant cytoplasmic staining of the lymphocytic allograft infiltrate was observed. These results provide a basis for using the anti-granzyme B antibodies for detection of cytotoxic cells in human tissues. The detection and quantitative analysis of the granzyme-associated cytotoxic cells may help to evaluate the significance of these functionally distinct cytotoxic cells in human tissues associated with increased expression of cytoplasmic granule effector molecules. | lld:pubmed |
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| pubmed-article:2024702 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2024702 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2024702 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2024702 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2024702 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2024702 | pubmed:month | May | lld:pubmed |
| pubmed-article:2024702 | pubmed:issn | 0002-9440 | lld:pubmed |
| pubmed-article:2024702 | pubmed:author | pubmed-author:TschoppJJ | lld:pubmed |
| pubmed-article:2024702 | pubmed:author | pubmed-author:PriceVV | lld:pubmed |
| pubmed-article:2024702 | pubmed:author | pubmed-author:TruongL DLD | lld:pubmed |
| pubmed-article:2024702 | pubmed:author | pubmed-author:HameedAA | lld:pubmed |
| pubmed-article:2024702 | pubmed:author | pubmed-author:KruhenbuhlOO | lld:pubmed |
| pubmed-article:2024702 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2024702 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:2024702 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2024702 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2024702 | pubmed:pagination | 1069-75 | lld:pubmed |
| pubmed-article:2024702 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2024702 | pubmed:meshHeading | pubmed-meshheading:2024702-... | lld:pubmed |
| pubmed-article:2024702 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2024702 | pubmed:articleTitle | Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues. | lld:pubmed |
| pubmed-article:2024702 | pubmed:affiliation | Department of Pathology, Methodist Hospital, Houston, Texas. | lld:pubmed |
| pubmed-article:2024702 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2024702 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2024702 | lld:pubmed |
