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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-6-4
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pubmed:abstractText |
Human granzyme B antigen is expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme B was generated using a prokaryotic expression vector under the control of T7 transcription and translation signals. The 25-kd recombinant protein (granzyme B) was used to develop a rabbit polyclonal antiserum. Purified anti-granzyme B antibodies were used to detect the antigen expression in cytotoxic cells in human tissues. Using the avidin-biotin-complex peroxidase technique, formalin-fixed, paraffin-embedded tissue sections from patients with acute mild or moderate allograft cardiac rejection were stained. A constant cytoplasmic staining of the lymphocytic allograft infiltrate was observed. These results provide a basis for using the anti-granzyme B antibodies for detection of cytotoxic cells in human tissues. The detection and quantitative analysis of the granzyme-associated cytotoxic cells may help to evaluate the significance of these functionally distinct cytotoxic cells in human tissues associated with increased expression of cytoplasmic granule effector molecules.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-1699101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2104899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2105060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2212674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2249651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2351567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2449086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2461088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2632370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2788710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2953813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-2960546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3008337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3042021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3076768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3126252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3257574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3262682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3263427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3280725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3292396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3419519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3536928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3537305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3755611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3887932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-3904772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-4069203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-6206131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-6352059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-6965393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2024702-7110326
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1069-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2024702-Antibodies,
pubmed-meshheading:2024702-Antigens,
pubmed-meshheading:2024702-Biopsy,
pubmed-meshheading:2024702-Granzymes,
pubmed-meshheading:2024702-Heart Transplantation,
pubmed-meshheading:2024702-Humans,
pubmed-meshheading:2024702-Immunohistochemistry,
pubmed-meshheading:2024702-Killer Cells, Natural,
pubmed-meshheading:2024702-Myocardium,
pubmed-meshheading:2024702-Serine Endopeptidases,
pubmed-meshheading:2024702-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:2024702-Transplantation, Homologous
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pubmed:year |
1991
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pubmed:articleTitle |
Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues.
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pubmed:affiliation |
Department of Pathology, Methodist Hospital, Houston, Texas.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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