|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1991-6-4
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
To better understand the structure and function of a baculovirus regulatory gene, the nucleotide sequence of IE-N expressed by Autographa californica nuclear polyhedrosis virus was determined. The 2.0-kb PstI-EcoRV restriction fragment (97.5 to 98.9 mu) encodes the upstream regulatory sequences, open reading frame, and downstream sequences of the immediate early IE-N gene. Using a convenient restriction site, the 285-bp promoter of IE-N was divided into two functional regions as defined by transient expression assays of mutant sequences. The sequences of IE-N from -1 to -45 nt encoded a minimal promoter capable of directing low levels of transcription. The minimal promoter was fully responsive to positive regulation by IE-N. The upstream region from -46 to -285 nt contains two direct repeats which increased levels of IE-N gene expression. Computer-assisted translation of the IE-N sequence indicates that this fragment of DNA encodes a single long open reading frame with a predicted molecular weight of 47,000. The amino acid sequence of the predicted protein exhibits three motifs common to transcriptional regulators: a serine-threonine rich region, a proline-rich region, and a polyglutamine tract. IE-N autoregulates its own expression and stimulates both IE-1 and IE-0 in transient assays. The stimulation of IE-1 may account for the augmenting activity of IE-N in the IE-1-mediated trans-activation of the 39K promoter.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0042-6822
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
182
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
279-86
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:2024466-Amino Acid Sequence,
pubmed-meshheading:2024466-Baculoviridae,
pubmed-meshheading:2024466-Base Sequence,
pubmed-meshheading:2024466-DNA, Viral,
pubmed-meshheading:2024466-DNA Mutational Analysis,
pubmed-meshheading:2024466-DNA-Binding Proteins,
pubmed-meshheading:2024466-Gene Expression Regulation, Viral,
pubmed-meshheading:2024466-Genes, Regulator,
pubmed-meshheading:2024466-Genes, Viral,
pubmed-meshheading:2024466-Molecular Sequence Data,
pubmed-meshheading:2024466-Oligonucleotides,
pubmed-meshheading:2024466-Promoter Regions, Genetic,
pubmed-meshheading:2024466-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2024466-Trans-Activators,
pubmed-meshheading:2024466-Viral Proteins,
pubmed-meshheading:2024466-Viral Structural Proteins
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Molecular analysis of a baculovirus regulatory gene.
|
|
pubmed:affiliation |
Departments of Biochemistry & Biophysics, Texas A&M University, College Station 77843.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
