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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1991-5-31
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pubmed:abstractText |
Sequence similarity between alpha B-crystallin and small heat shock proteins (HSPs) has prompted us to investigate whether alpha B-crystallin expression is induced by heat shock. Indeed, accumulation of alpha B-crystallin was detected immunologically in NIH 3T3 cells after incubation at elevated temperatures and after addition of Cd2+ or sodium arsenite to these cells. Two-dimensional gel electrophoresis revealed identity between alpha B-crystallin from eye lenses and from heat-treated fibroblasts. The promoter of the alpha B-crystallin gene was fused to the bacterial chloramphenicol acetyltransferase gene and was shown to confer heat inducibility on this reporter gene in transient transfection assays. A perfect heat shock element within the promoter region is likely to mediate this response. Small HSPs and alpha B-crystallin were shown to share the following two physical properties: (i) they form supramolecular structures with sedimentation values around 17 S and (ii) they are associated with the nucleus at high temperatures and are localized in the cytoplasm under normal conditions. We conclude that alpha B-crystallin has to be considered a member of the class of small HSPs.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3652-6
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:2023914-Animals,
pubmed-meshheading:2023914-Base Sequence,
pubmed-meshheading:2023914-Cell Compartmentation,
pubmed-meshheading:2023914-Cell Line,
pubmed-meshheading:2023914-Cell Nucleus,
pubmed-meshheading:2023914-Crystallins,
pubmed-meshheading:2023914-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:2023914-Gene Expression Regulation,
pubmed-meshheading:2023914-Heat-Shock Proteins,
pubmed-meshheading:2023914-Macromolecular Substances,
pubmed-meshheading:2023914-Mice,
pubmed-meshheading:2023914-Molecular Sequence Data,
pubmed-meshheading:2023914-Molecular Weight,
pubmed-meshheading:2023914-Promoter Regions, Genetic,
pubmed-meshheading:2023914-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:2023914-Transcription, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
Alpha B-crystallin is a small heat shock protein.
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pubmed:affiliation |
Division of Cancer Research, University of Zurich, Switzerland.
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pubmed:publicationType |
Journal Article
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