Linked Life Data

20236858

Source:http://linkedlifedata.com/resource/pubmed/id/20236858

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-4-2
pubmed:abstractText
The CdSe quantum dots (QDs) modified by mercapto-beta-cyclodextrin (CD) were synthesized and characterized by transmission electron microscopy, powder X-ray diffraction, excitation and emission spectra, and fluorescence lifetime. When lambda(ex)=370nm, the fluorescence peak of CdSe/CD QDs is at 525nm. Phenanthroline (Phen) is able to quench their fluorescence, which can be recovered by the addition of DNA. The quenching and restoration of fluorescence intensity were found to be linearly proportional to the amount of Phen and DNA, respectively. The variation of the fluorescence intensity of the CdSe/CD QDs-Phen system was studied, and it was demonstrated to result from a static mechanism due to the formation of a Phen inclusion complex with the CdSe QDs modified by mercapto-beta-cyclodextrin. The fluorescence recovery was due to the binding of DNA with Phen in the inclusion complex, leading to the freeing of the CdSe/CD QDs. The binding constants and sizes of the binding sites of the Phen-DNA interaction were calculated to be 1.33x10(7)mol(-1)L and 10.79bp.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-3557
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1617-23
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Recognition of DNA based on changes in the fluorescence intensity of CdSe/CD QDs-phenanthroline systems.
pubmed:affiliation
School of Chemistry and Environment, South China Normal University, Guangzhou 510631, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't