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pubmed-article:20230618pubmed:abstractTextThe episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP) and directed into a 2000 bp long matrix attachment region sequence (MARS) derived from the human beta-interferon gene. The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene expression.lld:pubmed
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pubmed-article:20230618pubmed:articleTitlepEPito: a significantly improved non-viral episomal expression vector for mammalian cells.lld:pubmed
pubmed-article:20230618pubmed:affiliationMax von Pettenkofer-Institute, University of Munich, Munich, Germany.lld:pubmed
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pubmed-article:20230618pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed