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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-3-29
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pubmed:abstractText |
The episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP) and directed into a 2000 bp long matrix attachment region sequence (MARS) derived from the human beta-interferon gene. The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene expression.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
1472-6750
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20
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pubmed:dateRevised |
2010-9-30
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pubmed:meshHeading |
pubmed-meshheading:20230618-Animals,
pubmed-meshheading:20230618-CpG Islands,
pubmed-meshheading:20230618-Cytomegalovirus,
pubmed-meshheading:20230618-Gene Expression,
pubmed-meshheading:20230618-Genetic Vectors,
pubmed-meshheading:20230618-Humans,
pubmed-meshheading:20230618-Liver,
pubmed-meshheading:20230618-Male,
pubmed-meshheading:20230618-Matrix Attachment Regions,
pubmed-meshheading:20230618-Mice,
pubmed-meshheading:20230618-NIH 3T3 Cells,
pubmed-meshheading:20230618-Plasmids,
pubmed-meshheading:20230618-Promoter Regions, Genetic,
pubmed-meshheading:20230618-Replicon,
pubmed-meshheading:20230618-Transfection,
pubmed-meshheading:20230618-Transgenes
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pubmed:year |
2010
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pubmed:articleTitle |
pEPito: a significantly improved non-viral episomal expression vector for mammalian cells.
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pubmed:affiliation |
Max von Pettenkofer-Institute, University of Munich, Munich, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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