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rdf:type |
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lifeskim:mentions |
umls-concept:C0005495,
umls-concept:C0220781,
umls-concept:C0332256,
umls-concept:C0560175,
umls-concept:C0887838,
umls-concept:C1180347,
umls-concept:C1412504,
umls-concept:C1419227,
umls-concept:C1422187,
umls-concept:C1706209,
umls-concept:C1707511,
umls-concept:C1710082
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pubmed:issue |
4
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pubmed:dateCreated |
2010-4-2
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pubmed:abstractText |
Actin dynamics is a tightly regulated process involved in various cellular events including biogenesis of clathrin-coated, AP-1 (adaptor protein 1)-coated transport carriers connecting the trans-Golgi network (TGN) and the endocytic pathway. However, the mechanisms coordinating coat assembly, membrane and actin remodelling during post-TGN transport remain poorly understood. Here we show that the Arf1 (ADP-ribosylation factor 1) GTPase synchronizes the TGN association of clathrin-AP-1 coats and protein complexes comprising CYFIP (cytoplasmic fragile-X mental retardation interacting protein; Sra, PIR121), a clathrin heavy chain binding protein associated with mental retardation. The Rac1 GTPase and its exchange factor beta-PIX (PAK-interacting exchange factor) activate these complexes, allowing N-WASP-dependent and Arp2/3-dependent actin polymerization towards membranes, thus promoting tubule formation. These phenomena can be recapitulated with synthetic membranes. This protein-network-based mechanism facilitates the sequential coordination of Arf1-dependent membrane priming, through the recruitment of coats and CYFIP-containing complexes, and of Rac1-dependent actin polymerization, and provides complementary but independent levels of regulation during early stages of clathrin-AP1-coated carrier biogenesis.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-Ribosylation Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Actin-Related Protein 2-3 Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Protein Complex 1,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/CYFIP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CYFIP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/RAC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/WASL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wiskott-Aldrich Syndrome Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/rac1 GTP-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/rho guanine nucleotide exchange...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1476-4679
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
330-40
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pubmed:dateRevised |
2010-5-5
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pubmed:meshHeading |
pubmed-meshheading:20228810-ADP-Ribosylation Factor 1,
pubmed-meshheading:20228810-Actin-Related Protein 2-3 Complex,
pubmed-meshheading:20228810-Actins,
pubmed-meshheading:20228810-Adaptor Protein Complex 1,
pubmed-meshheading:20228810-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:20228810-Animals,
pubmed-meshheading:20228810-Brain,
pubmed-meshheading:20228810-COS Cells,
pubmed-meshheading:20228810-Cell Fractionation,
pubmed-meshheading:20228810-Cercopithecus aethiops,
pubmed-meshheading:20228810-Clathrin-Coated Vesicles,
pubmed-meshheading:20228810-Endocytosis,
pubmed-meshheading:20228810-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:20228810-HeLa Cells,
pubmed-meshheading:20228810-Humans,
pubmed-meshheading:20228810-Models, Biological,
pubmed-meshheading:20228810-Protein Transport,
pubmed-meshheading:20228810-RNA Interference,
pubmed-meshheading:20228810-Swine,
pubmed-meshheading:20228810-Time Factors,
pubmed-meshheading:20228810-Transfection,
pubmed-meshheading:20228810-Wiskott-Aldrich Syndrome Protein, Neuronal,
pubmed-meshheading:20228810-rac1 GTP-Binding Protein,
pubmed-meshheading:20228810-trans-Golgi Network
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pubmed:year |
2010
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pubmed:articleTitle |
Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN.
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pubmed:affiliation |
Biotechnology Center, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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