| pubmed-article:202258 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0017968 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:202258 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:202258 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:202258 | pubmed:dateCreated | 1978-2-18 | lld:pubmed |
| pubmed-article:202258 | pubmed:abstractText | 1. Confluent human skin fibroblasts maintained in a chemically defined medium incorporate l-[1-(3)H]fucose in a linear manner with time into non-diffusible macromolecules for up to 48h. Chromatographic analysis demonstrated that virtually all the macromolecule-associated (3)H was present as [(3)H]fucose. 2. Equilibrium CsCl-density-gradient centrifugation established that [(3)H]fucose-labelled macromolecules released into the medium were predominantly glycoproteins. Confirmation of this finding was provided by molecular-size analyses of the [(3)H]fucose-labelled material before and after trypsin digestion. 3. The [(3)H]fucose-labelled glycoproteins released into fibroblast culture medium were analysed by gel-filtration chromatography and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. These techniques demonstrated that the major fucosylated glycoprotein had an apparent mol.wt. of 230000-250000; several minor labelled species were also detected. 4. Dual-labelling experiments with [(3)H]fucose and (14)C-labelled amino acids indicated that the major fucosylated glycoprotein was synthesized de novo by cultured fibroblasts. The non-collagenous nature of this glycoprotein was established by three independent methods. 5. Gel-filtration analysis before and after reduction with dithiothreitol showed that the major glycoprotein occurs as a disulphide-bonded dimer when analysed under denaturing conditions. Further experiments demonstrated that this glycoprotein was the predominant labelled species released into the medium when fibroblasts were incubated with [(35)S]cysteine. 6. The relationship between the major fucosylated glycoprotein and a glycoprotein, or group of glycoproteins, variously known as fibronectin, LETS protein, cell-surface protein etc., is discussed. | lld:pubmed |
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| pubmed-article:202258 | pubmed:language | eng | lld:pubmed |
| pubmed-article:202258 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:202258 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:202258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:202258 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:202258 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:202258 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:202258 | pubmed:author | pubmed-author:JacksonD SDS | lld:pubmed |
| pubmed-article:202258 | pubmed:author | pubmed-author:GrantM EME | lld:pubmed |
| pubmed-article:202258 | pubmed:author | pubmed-author:SearC HCH | lld:pubmed |
| pubmed-article:202258 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:202258 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:202258 | pubmed:volume | 168 | lld:pubmed |
| pubmed-article:202258 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:202258 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:202258 | pubmed:pagination | 91-103 | lld:pubmed |
| pubmed-article:202258 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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