|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0016030,
umls-concept:C0017968,
umls-concept:C0030685,
umls-concept:C0086418,
umls-concept:C0220781,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C1123023,
umls-concept:C1283071,
umls-concept:C1963578
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1978-2-18
|
|
pubmed:abstractText |
1. Confluent human skin fibroblasts maintained in a chemically defined medium incorporate l-[1-(3)H]fucose in a linear manner with time into non-diffusible macromolecules for up to 48h. Chromatographic analysis demonstrated that virtually all the macromolecule-associated (3)H was present as [(3)H]fucose. 2. Equilibrium CsCl-density-gradient centrifugation established that [(3)H]fucose-labelled macromolecules released into the medium were predominantly glycoproteins. Confirmation of this finding was provided by molecular-size analyses of the [(3)H]fucose-labelled material before and after trypsin digestion. 3. The [(3)H]fucose-labelled glycoproteins released into fibroblast culture medium were analysed by gel-filtration chromatography and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. These techniques demonstrated that the major fucosylated glycoprotein had an apparent mol.wt. of 230000-250000; several minor labelled species were also detected. 4. Dual-labelling experiments with [(3)H]fucose and (14)C-labelled amino acids indicated that the major fucosylated glycoprotein was synthesized de novo by cultured fibroblasts. The non-collagenous nature of this glycoprotein was established by three independent methods. 5. Gel-filtration analysis before and after reduction with dithiothreitol showed that the major glycoprotein occurs as a disulphide-bonded dimer when analysed under denaturing conditions. Further experiments demonstrated that this glycoprotein was the predominant labelled species released into the medium when fibroblasts were incubated with [(35)S]cysteine. 6. The relationship between the major fucosylated glycoprotein and a glycoprotein, or group of glycoproteins, variously known as fibronectin, LETS protein, cell-surface protein etc., is discussed.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1059102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1092340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1097576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1117000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1126522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1140390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-123485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-1275503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-13971270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-14247639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-14297872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-173224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-173226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-176004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-177379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-177979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-188411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-189273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4127103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4186295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4205351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4272599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4280264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4326772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4353889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4361679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4407489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4509328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4530317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4570356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-4746935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5056627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5126913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5158921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5276307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5449186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-556940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5650855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-56527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5660634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5783840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-5959433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-6033547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-6037551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-779852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-819259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-902855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-958404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/202258-962927
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
168
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
91-103
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:202258-Humans,
pubmed-meshheading:202258-Ascorbic Acid,
pubmed-meshheading:202258-Skin,
pubmed-meshheading:202258-Cysteine,
pubmed-meshheading:202258-Fucose,
pubmed-meshheading:202258-Molecular Weight,
pubmed-meshheading:202258-Glycoproteins,
pubmed-meshheading:202258-Fibroblasts,
pubmed-meshheading:202258-Cells, Cultured,
pubmed-meshheading:202258-Macromolecular Substances,
pubmed-meshheading:202258-Microbial Collagenase
|
