Linked Life Data

2022330

Source:http://linkedlifedata.com/resource/pubmed/id/2022330

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-5-31
pubmed:databankReference
pubmed:abstractText
AmU1, a DNA fragment containing a U1 small nuclear RNA (snRNA)-encoding gene, was isolated from the axolotl, Ambystoma mexicanum. Although this U1 snRNA, produced in axolotl oocytes, exhibits the lowest degree of sequence conservation among vertebrates, its secondary structure is maintained by a number of compensatory base changes. The proximal sequence element (PSE) is only weakly similar to that of the previously characterized Xenopus laevis PSE. Exchanging either the entire upstream regions with their X. laevis U1 (XlU1) homologues or only the PSE with the XlU1 PSE increases the transcription rate of the AmU1 gene to a level similar to that of the XlU1 gene. However, while allowing the AmU1 gene to be transcribed with high efficiency in X. laevis oocytes, the strict swapping of the 12-bp constituting the XlU1 PSE does not confer competitive ability to the AmU1 gene. We present evidence that the PSE is the major, but not the only element responsible for the low template activity of the AmU1 gene in X. laevis oocytes and our data suggest that other sequences, perhaps flanking the PSE, might also influence the binding of factor(s) participating in the assembly of the transcription complex.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Sequence, organization and transcriptional analysis of a gene encoding a U1 snRNA from the axolotl, Ambystoma mexicanum.
pubmed:affiliation
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't