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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2010-3-12
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pubmed:abstractText |
An understanding of sleep requires the identification of distinct cellular circuits that mediate the action of specific sleep:wake-regulating molecules, but such analysis has been very limited. We identify here a circuit that underlies the wake-promoting effects of octopamine in Drosophila. Using MARCM, we identified the ASM cells in the medial protocerebrum as the wake-promoting octopaminergic cells. We then blocked octopamine signaling in random areas of the fly brain and mapped the postsynaptic effect to insulin-secreting neurons of the pars intercerebralis (PI). These PI neurons show altered potassium channel function as well as an increase in cAMP in response to octopamine, and genetic manipulation of their electrical excitability alters sleep:wake behavior. Effects of octopamine on sleep:wake are mediated by the cAMP-dependent isoform of the OAMB receptor. These studies define the cellular and molecular basis of octopamine action and suggest that the PI is a sleep:wake-regulating neuroendocrine structure like the mammalian hypothalamus.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/OAMB protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Octopamine,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter,
http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1097-4199
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
11
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
670-81
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:20223202-Adrenergic alpha-Agonists,
pubmed-meshheading:20223202-Animals,
pubmed-meshheading:20223202-Animals, Genetically Modified,
pubmed-meshheading:20223202-Behavior, Animal,
pubmed-meshheading:20223202-Circadian Rhythm,
pubmed-meshheading:20223202-Cyclic AMP,
pubmed-meshheading:20223202-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:20223202-Drosophila,
pubmed-meshheading:20223202-Drosophila Proteins,
pubmed-meshheading:20223202-Electric Stimulation,
pubmed-meshheading:20223202-Green Fluorescent Proteins,
pubmed-meshheading:20223202-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:20223202-Membrane Potentials,
pubmed-meshheading:20223202-Nerve Net,
pubmed-meshheading:20223202-Neurons,
pubmed-meshheading:20223202-Octopamine,
pubmed-meshheading:20223202-Patch-Clamp Techniques,
pubmed-meshheading:20223202-Potassium Channel Blockers,
pubmed-meshheading:20223202-Receptors, Neurotransmitter,
pubmed-meshheading:20223202-Sleep,
pubmed-meshheading:20223202-Tetraethylammonium,
pubmed-meshheading:20223202-Wakefulness
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pubmed:year |
2010
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pubmed:articleTitle |
Identification of a neural circuit that underlies the effects of octopamine on sleep:wake behavior.
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pubmed:affiliation |
Department of Neuroscience, University of Pennsylvania Medical School, Philadelphia, PA 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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