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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2010-4-30
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pubmed:abstractText |
Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRNA modulator of gene expressioN) vector technology is based on snoRNA HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets. Gene-specific knockdowns are demonstrated for endogenous cellular proteins and for G/YFP-fusion proteins. Multiplex snoMEN vectors coexpress multiple snoRNAs in one transcript, targeted either to different genes or to different sites in the same gene. Protein replacement snoMEN vectors can express a single transcript combining cDNA for a tagged protein with introns containing cognate snoRNAs targeted to knockdown the endogenous cellular protein. We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20219969-10395551,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20219969-9844635
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1939-4586
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1569-84
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pubmed:dateRevised |
2010-9-30
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pubmed:meshHeading |
pubmed-meshheading:20219969-Cell Nucleolus,
pubmed-meshheading:20219969-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:20219969-Cloning, Molecular,
pubmed-meshheading:20219969-Gene Expression,
pubmed-meshheading:20219969-Gene Knockdown Techniques,
pubmed-meshheading:20219969-Gene Library,
pubmed-meshheading:20219969-Genetic Vectors,
pubmed-meshheading:20219969-Green Fluorescent Proteins,
pubmed-meshheading:20219969-HeLa Cells,
pubmed-meshheading:20219969-Humans,
pubmed-meshheading:20219969-In Situ Hybridization, Fluorescence,
pubmed-meshheading:20219969-Microscopy, Fluorescence,
pubmed-meshheading:20219969-Mutation,
pubmed-meshheading:20219969-RNA, Small Nucleolar,
pubmed-meshheading:20219969-RNA Interference,
pubmed-meshheading:20219969-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20219969-Survival of Motor Neuron 1 Protein,
pubmed-meshheading:20219969-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
Analysis of human small nucleolar RNAs (snoRNA) and the development of snoRNA modulator of gene expression vectors.
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pubmed:affiliation |
Wellcome Trust Centre for Gene Regulation and Expression, and Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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