Linked Life Data

20218715

Source:http://linkedlifedata.com/resource/pubmed/id/20218715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2010-3-31
pubmed:abstractText
Quantum dots (QDs) are a powerful alternative to organic dyes and fluorescent proteins for biological and biomedical applications. These semiconductor nanocrystals are traditionally synthesized above 200 degrees C in organic solvents using toxic and costly precursors, and further steps are required to conjugate them to a biological ligand. Here, we describe a simple, aqueous route for the one-pot synthesis of antibody-derivatized zinc sulfide (ZnS) immuno-QDs. In this strategy, easily expressed and purified fusion proteins perform the dual function of nanocrystal mineralizers through ZnS binding sequences identified by cell surface display and adaptors for immunoglobin G (IgG) conjugation through a tandem repeat of the B domain of Staphylococcus aureus protein A. Although approximately 4.3 nm ZnS wurtzite cores could be biomineralized from either zinc chloride or zinc acetate precursors, only the latter salt gives rise to protein-coated QDs with long shelf life and narrow hydrodynamic diameters (8.8 +/- 1.4 nm). The biofabricated QDs have a quantum yield of 2.5% and blue-green ensemble emission with contributions from the band-edge at 340 nm and from trap states at 460 and 665 nm that are influenced by the identity of the protein shell. Murine IgG(1) antibodies exhibit high affinity (K(d) = 60 nM) for the protein shell, and stable immuno-QDs with a hydrodynamic diameter of 14.1 +/- 1.3 nm are readily obtained by mixing biofabricated nanocrystals with human IgG.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4731-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Single-pot biofabrication of zinc sulfide immuno-quantum dots.
pubmed:affiliation
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, Washington 98195, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't