Linked Life Data

20218004

Source:http://linkedlifedata.com/resource/pubmed/id/20218004

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-3-11
pubmed:abstractText
Isoelectric focussing (IEF) in 6M urea/4% polyacrylamide gel of reduced human fibrinogen and the fibrins formed by reptilase and thrombin gave a complex multibanded pattern. Similar results were obtained on IEF in urea/agarose gels containing excess mercaptoethanol. To characterize these multiple bands on the basis of molecular weight secondary electrophoreses into a discontinuous SDS-polyacrylamide gel system were carried out perpendicular to the initial IEF's. At least five major Alpha chains ranging in pI from 7.14 to 7.60 were found in fibrinogen. These were shifted 0.40 pI units towards the cathode after the action of reptilase or thrombin due to the release of electronegative fibrinopeptide A species. A similar change occurred with the three main B beta chains with initial pI's of 6.81 to 7.13, but only after thrombin action. Gamma dimer showed no significant difference in pI from its component chains 6.05 to 6.17.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
489-97
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Analysis of reptilase and thrombin-induced changes in fibrinogen subunits by isoelectric focussing.
pubmed:affiliation
Haematology Department, Royal Prince Alfred Hospital, Sydney, Australia.
pubmed:publicationType
Journal Article