Source:http://linkedlifedata.com/resource/pubmed/id/20217638
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
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| pubmed:dateCreated |
2010-9-13
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| pubmed:abstractText |
The compulsive gnawing (CG) test has been used for numerous years as an assay to determine the dopaminergic activity of various compounds. We developed a new method of quantification via a digitization step which allowed a more precise measurement of the gnawing activity. It was the aim of the present study to explore possible dopaminergic effects of salvinorin A (SA), the major active compound of Salvia divinorum, using the new digitized CG test. A group of experiments using male C57BL/6 mice were performed to validate the new method of quantification showing only significant increases of gnawing when the dopamine reuptake inhibitors buproprion (20 mg/kg, p.0.) and nomifensine (10?mg/kg, i.p.) were given concomitantly with apomorphine (10?mg/kg, i.p.). Different concentrations of the SA (1.0, 2.5, 5, and 10?mg/kg, i.p.) were tested with positive dopaminergic activity when administered with apomorphine which differed from the semisynthetic counterpart U-69593. Furthermore, the activity observed with SA was unsuccessfully antagonized by the ?-opioid receptor antagonist norbinaltorphimine (NorBNI; 10 and 20?mg/kg, i.p.), while the dopamine antagonist haloperidol did successfully block (0.06?mg/kg, i.p.) the gnawing activity seen with SA. Our data further strengthen the argument that salvinorin A is not a selective ?-opioid receptor agonist and is the first in vivo study that veers from salvinorin A acting solely like its synthetic counterparts. Furthermore, the digitized CG test system used in this study provides a new computational method to accurately detect behavior associated with dopaminergic neurotransmission.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes, Clerodane,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Uptake Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa,
http://linkedlifedata.com/resource/pubmed/chemical/salvinorin A
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1439-0221
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| pubmed:author | |
| pubmed:copyrightInfo |
© Georg Thieme Verlag KG Stuttgart-New York.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
76
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1405-10
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| pubmed:meshHeading |
pubmed-meshheading:20217638-Analog-Digital Conversion,
pubmed-meshheading:20217638-Animals,
pubmed-meshheading:20217638-Behavior, Animal,
pubmed-meshheading:20217638-Compulsive Behavior,
pubmed-meshheading:20217638-Disease Models, Animal,
pubmed-meshheading:20217638-Diterpenes, Clerodane,
pubmed-meshheading:20217638-Dopamine Agonists,
pubmed-meshheading:20217638-Dopamine Uptake Inhibitors,
pubmed-meshheading:20217638-Dose-Response Relationship, Drug,
pubmed-meshheading:20217638-Male,
pubmed-meshheading:20217638-Mastication,
pubmed-meshheading:20217638-Mice,
pubmed-meshheading:20217638-Mice, Inbred C57BL,
pubmed-meshheading:20217638-Motor Activity,
pubmed-meshheading:20217638-Narcotic Antagonists,
pubmed-meshheading:20217638-Plant Extracts,
pubmed-meshheading:20217638-Receptors, Opioid, kappa,
pubmed-meshheading:20217638-Salvia
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| pubmed:year |
2010
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| pubmed:articleTitle |
A new digitized method of the compulsive gnawing test revealed dopaminergic activity of salvinorin A in vivo.
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| pubmed:affiliation |
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, Florida 32610-0494, USA.
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| pubmed:publicationType |
Journal Article,
Validation Studies
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