Linked Life Data

20213949

Source:http://linkedlifedata.com/resource/pubmed/id/20213949

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-3-9
pubmed:abstractText
Affinity chromatography is very useful in the investigation and characterization of specific interaction between biomolecules. Frontal analysis in affinity chromatography is advantageous from both theoretical and experimental viewpoints. The theory is very simple because we can describe this system by means of a simple equilibrium problem.Chromatographic data can be related easily to the amount of interacting molecules and the equilibrium constant. Useful equations analogous to those of enzyme kinetics can also be derived easily. Thus, frontal affinity chromatography provides information almost identical to that obtainable by enzyme kinetic studies. In addition, this method is more general because it does not depend on enzymatic activity. Experiment is very easy and does not require any special equipment. It is a powerful tool, especially for complicated systems where it has been difficult to find an appropriate method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
376
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-47
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan.
pubmed:publicationType
Journal Article