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rdf:type |
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lifeskim:mentions |
umls-concept:C0021469,
umls-concept:C0030281,
umls-concept:C0031715,
umls-concept:C0072980,
umls-concept:C0167117,
umls-concept:C1150587,
umls-concept:C1280500,
umls-concept:C1366876,
umls-concept:C1367731,
umls-concept:C1511636,
umls-concept:C1705632,
umls-concept:C1833235
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pubmed:issue |
5
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pubmed:dateCreated |
2010-5-4
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pubmed:abstractText |
It has been reported that the immunosuppressant rapamycin decreases the viability of pancreatic beta cells. In contrast, exendin-4, an analogue of glucagon-like peptide-1, has been found to inhibit beta cell death and to increase beta cell mass. We investigated the effects of exendin-4 on the cytotoxic effect of rapamycin in beta cells. Incubation with 10 nM rapamycin induced cell death in 12 h in murine beta cell line MIN6 cells and Wistar rat islets, but not when coincubated with 10 nM exendin-4. Rapamycin was found to increase phosphorylation of c-Jun amino-terminal kinase (JNK) and p38 in 30 minutes in MIN6 cells and Wistar rat islets while exendin-4 decreased their phosphorylation. Akt and extracellular signal-regulated kinase (ERK) were not involved in the cytoprotective effect of exendin-4. These results indicate that exendin-4 may exert its protective effect against rapamycin-induced cell death in pancreatic beta cells by inhibiting JNK and p38 signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Sirolimus,
http://linkedlifedata.com/resource/pubmed/chemical/Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/exenatide,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1439-4286
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pubmed:author |
pubmed-author:HamasakiAA,
pubmed-author:HaradaNN,
pubmed-author:HarashimaSS,
pubmed-author:InagakiNN,
pubmed-author:KawasakiYY,
pubmed-author:MukaiEE,
pubmed-author:NakamuraYY,
pubmed-author:SasakiMM,
pubmed-author:SeinoYY,
pubmed-author:ToyodaKK,
pubmed-author:YamadaCC,
pubmed-author:YamadaYY,
pubmed-author:YamaneSS
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pubmed:copyrightInfo |
Georg Thieme Verlag KG Stuttgart-New York.
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pubmed:issnType |
Electronic
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20213584-Animals,
pubmed-meshheading:20213584-Antibiotics, Antineoplastic,
pubmed-meshheading:20213584-Blotting, Western,
pubmed-meshheading:20213584-Cell Death,
pubmed-meshheading:20213584-Cell Survival,
pubmed-meshheading:20213584-Culture Media,
pubmed-meshheading:20213584-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:20213584-Dose-Response Relationship, Drug,
pubmed-meshheading:20213584-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20213584-Flow Cytometry,
pubmed-meshheading:20213584-Insulin,
pubmed-meshheading:20213584-Insulin-Secreting Cells,
pubmed-meshheading:20213584-Islets of Langerhans,
pubmed-meshheading:20213584-MAP Kinase Kinase 4,
pubmed-meshheading:20213584-Peptides,
pubmed-meshheading:20213584-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:20213584-Phosphorylation,
pubmed-meshheading:20213584-Rats,
pubmed-meshheading:20213584-Rats, Wistar,
pubmed-meshheading:20213584-Sirolimus,
pubmed-meshheading:20213584-Venoms,
pubmed-meshheading:20213584-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2010
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pubmed:articleTitle |
Exendin-4 protects pancreatic beta cells from the cytotoxic effect of rapamycin by inhibiting JNK and p38 phosphorylation.
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pubmed:affiliation |
Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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