Linked Life Data

20211157

Source:http://linkedlifedata.com/resource/pubmed/id/20211157

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-4-19
pubmed:abstractText
Oxidative and carbonyl stress are detrimental in the pathogenesis of diabetic complications, as well as in other chronic diseases. However, this process may be decreased by dietary bioactive compounds. Almond skin is an abundant source of bioactive compounds and antioxidants, including polyphenolic flavonoids, which may contribute to the decrease in oxidative and carbonyl stress. In this study, four Almond Skin Extracts (ASEI, ASEII, ASEIII, and ASEIV) were prepared by different methods and evaluated for their antioxidant activity. The order of the polyphenol content (total muM gallic acid equivalents) of the four extracts was found to be, in decreasing order of effectiveness: ASEI>ASEIII>ASEIV>ASEII. The order of Ferric-reducing antioxidant power (FRAP, microM FeSO(4)/g) value, in decreasing order was ASEI (216)>ASEIII (176)>ASEIV (89)>ASEII (85). The order of ASE effectiveness for decreasing protein carbonyation induced by the copper Fenton reaction was ASEI>ASEIV>ASEII>ASEIII. The order of antioxidant effectiveness for inhibiting tertiary-butyl hydroperoxide (TBH) induced microsomal lipid peroxidation was ASEI>ASEIV>ASEII, ASEIII. Also, the order of ASE effectiveness for inhibiting TBH induced hepatocyte cell death was: ASEIII, ASEIV>ASEI, ASEII. Catechin also protected hepatocytes from TBH induced hepatocyte, lipid peroxidation and cytotoxicity. In a cell free model, equimolar concentrations of catechin or epicatechin rescued serum albumin from protein carbonylation induced by methylglyoxal (MGO). Catechin, epicatechin and ASEI all decreased gloxal induced hepatocyte cell death and reactive oxygen species (ROS) formation in GSH-depleted hepatocytes. Catechin and epicatechin protected against GO or MGO induced hepatocyte cell death, protein carbonylation and ROS formation. Catechin was more effective than epicatechin. Our results suggest that (a) bioactive almond skin constituents in the non-lipophilic polyphenol extract were the most effective at protecting hepatocytes against hydroperoxide induced hepatocyte oxidative stress and in protecting against dicarbonyl induced cytotoxicity; (b) catechins, the major polyphenol in the extract, were also effective at preventing GO or MGO cytotoxicity likely by trapping GO and MGO and/or rescuing hepatocytes from protein carbonylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1872-7786
pubmed:author
pubmed:copyrightInfo
2010 Elsevier Ireland Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
29
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-9
pubmed:meshHeading
pubmed-meshheading:20211157-Animals, pubmed-meshheading:20211157-Antioxidants, pubmed-meshheading:20211157-Catechin, pubmed-meshheading:20211157-Cytoprotection, pubmed-meshheading:20211157-Dose-Response Relationship, Drug, pubmed-meshheading:20211157-Drug-Induced Liver Injury, pubmed-meshheading:20211157-Ferric Compounds, pubmed-meshheading:20211157-Glutathione, pubmed-meshheading:20211157-Glyoxal, pubmed-meshheading:20211157-Hepatocytes, pubmed-meshheading:20211157-Hydrogen Peroxide, pubmed-meshheading:20211157-Lipid Peroxidation, pubmed-meshheading:20211157-Oxidative Stress, pubmed-meshheading:20211157-Phytotherapy, pubmed-meshheading:20211157-Plant Extracts, pubmed-meshheading:20211157-Protective Agents, pubmed-meshheading:20211157-Protein Carbonylation, pubmed-meshheading:20211157-Prunus, pubmed-meshheading:20211157-Pyruvaldehyde, pubmed-meshheading:20211157-Rats, pubmed-meshheading:20211157-Rats, Sprague-Dawley, pubmed-meshheading:20211157-Reactive Oxygen Species, pubmed-meshheading:20211157-Time Factors, pubmed-meshheading:20211157-tert-Butylhydroperoxide
pubmed:year
2010
pubmed:articleTitle
Cytoprotection by almond skin extracts or catechins of hepatocyte cytotoxicity induced by hydroperoxide (oxidative stress model) versus glyoxal or methylglyoxal (carbonylation model).
pubmed:affiliation
Northwest A & F University, Yangling, Shaanxi, China.
pubmed:publicationType
Journal Article, Comparative Study