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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-4-2
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pubmed:abstractText |
Several subunits of the class III phosphatidylinositol-3-OH kinase (PI(3)K-III) complex are known as tumour suppressors. Here we uncover a function for this complex and its catalytic product phosphatidylinositol-3-phosphate (PtdIns(3)P) in cytokinesis. We show that PtdIns(3)P localizes to the midbody during cytokinesis and recruits a centrosomal protein, FYVE-CENT (ZFYVE26), and its binding partner TTC19, which in turn interacts with CHMP4B, an endosomal sorting complex required for transport (ESCRT)-III subunit implicated in the abscission step of cytokinesis. Translocation of FYVE-CENT and TTC19 from the centrosome to the midbody requires another FYVE-CENT-interacting protein, the microtubule motor KIF13A. Depletion of the VPS34 or Beclin 1 subunits of PI(3)K-III causes cytokinesis arrest and an increased number of binucleate and multinucleate cells, in a similar manner to the depletion of FYVE-CENT, KIF13A or TTC19. These results provide a mechanism for the translocation and docking of a cytokinesis regulatory machinery at the midbody.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/BECN1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CHMP4B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endosomal Sorting Complexes...,
http://linkedlifedata.com/resource/pubmed/chemical/KIF13A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylinositol 3-phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/spastizin protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1476-4679
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
362-71
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:20208530-Animals,
pubmed-meshheading:20208530-Apoptosis Regulatory Proteins,
pubmed-meshheading:20208530-Biosensing Techniques,
pubmed-meshheading:20208530-Carrier Proteins,
pubmed-meshheading:20208530-Cell Cycle,
pubmed-meshheading:20208530-Centrosome,
pubmed-meshheading:20208530-Cytokinesis,
pubmed-meshheading:20208530-Drosophila,
pubmed-meshheading:20208530-Drosophila Proteins,
pubmed-meshheading:20208530-Endosomal Sorting Complexes Required for Transport,
pubmed-meshheading:20208530-HeLa Cells,
pubmed-meshheading:20208530-Humans,
pubmed-meshheading:20208530-Kinesin,
pubmed-meshheading:20208530-Membrane Proteins,
pubmed-meshheading:20208530-Mice,
pubmed-meshheading:20208530-Microscopy, Fluorescence,
pubmed-meshheading:20208530-Mitosis,
pubmed-meshheading:20208530-Mitotic Spindle Apparatus,
pubmed-meshheading:20208530-Mutation,
pubmed-meshheading:20208530-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:20208530-Phosphatidylinositol Phosphates,
pubmed-meshheading:20208530-Protein Binding,
pubmed-meshheading:20208530-Protein Transport,
pubmed-meshheading:20208530-RNA Interference,
pubmed-meshheading:20208530-Recombinant Fusion Proteins,
pubmed-meshheading:20208530-Signal Transduction,
pubmed-meshheading:20208530-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody.
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pubmed:affiliation |
Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N-0310 Oslo, Norway.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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