| pubmed-article:20206951 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20206951 | lifeskim:mentions | umls-concept:C0330555 | lld:lifeskim |
| pubmed-article:20206951 | lifeskim:mentions | umls-concept:C0205474 | lld:lifeskim |
| pubmed-article:20206951 | lifeskim:mentions | umls-concept:C0023115 | lld:lifeskim |
| pubmed-article:20206951 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:20206951 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:20206951 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:20206951 | pubmed:dateCreated | 2010-4-7 | lld:pubmed |
| pubmed-article:20206951 | pubmed:abstractText | A protease, which we designate Eumiliin, was isolated from the latex of Euphorbia milii var. hislopii by a combination of ion-exchange chromatographic steps using DEAE-Sephacel and gel-filtration with Sephadex G-75. Eumiliin is a monomeric protein with an apparent molecular mass of 30 kDa by SDS-PAGE under reducing conditions and gave one main peak at 29,814 KDa in MALDI-TOF/TOF mass spectrometry. Eumiliin has caseinolytic and fibrinogenolytic activities, but no hemorrhagic or defibrinating activities. The enzyme readily hydrolyzes the Aalpha-chain of fibrinogen and, more slowly, the Bbeta-chain. Its fibrinogenolytic activity is inhibited by beta-mercaptoethanol and leupeptin. In contrast, EDTA and benzamidine did not affect the activity of Eumiliin. The caseinolytic activity of Eumiliin had a pH optimum of 8.0 and was stable in solution at up to 40 degrees C; activity was completely lost at >or=80 degrees C. Intraplantar injection of Eumiliin (1-25 microg/paw) caused a dose- and time-dependent hyperalgesia, which peaked 1-5h after enzyme injection. Intraplantar injection of Eumiliin (1-25 microg/paw) also caused an oedematogenic response that was maximal after 1h. Morphological analyses indicated that Eumiliin induced an intense myonecrosis, with visible leukocyte infiltrate and damaged muscle cells 24h after injection. | lld:pubmed |
| pubmed-article:20206951 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20206951 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20206951 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20206951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20206951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20206951 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20206951 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20206951 | pubmed:month | May | lld:pubmed |
| pubmed-article:20206951 | pubmed:issn | 1873-3700 | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:SilvaM CMC | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:CostaJ OJO | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:OliveiraFF | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:Penha-SilvaNN | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:GomesM SMS | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:BelettiM EME | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:FonsecaK CKC | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:MoraisN C GNC | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:QueirozM RMR | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:MamedeC C NCC | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:TorresF SFS | lld:pubmed |
| pubmed-article:20206951 | pubmed:author | pubmed-author:CanabravaH... | lld:pubmed |
| pubmed-article:20206951 | pubmed:copyrightInfo | Copyright 2010 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20206951 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20206951 | pubmed:volume | 71 | lld:pubmed |
| pubmed-article:20206951 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20206951 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20206951 | pubmed:pagination | 708-15 | lld:pubmed |
| pubmed-article:20206951 | pubmed:meshHeading | pubmed-meshheading:20206951... | lld:pubmed |
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| pubmed-article:20206951 | pubmed:meshHeading | pubmed-meshheading:20206951... | lld:pubmed |
| pubmed-article:20206951 | pubmed:meshHeading | pubmed-meshheading:20206951... | lld:pubmed |
| pubmed-article:20206951 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20206951 | pubmed:articleTitle | Purification and biochemical characterization of Eumiliin from Euphorbia milii var. hislopii latex. | lld:pubmed |
| pubmed-article:20206951 | pubmed:affiliation | Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. | lld:pubmed |
| pubmed-article:20206951 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20206951 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
