Source:http://linkedlifedata.com/resource/pubmed/id/20203153
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-3-24
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| pubmed:abstractText |
Citrate carrier (CiC), an integral protein of the mitochondrial inner membrane, plays an important role in hepatic intermediary metabolism, supplying the cytosol with acetyl-coenzyme A for fatty acid and cholesterol synthesis. Here, the effect of streptozotocin-induced diabetes on CiC activity and expression in rat liver was investigated. The rate of citrate transport was reduced by about 35% in mitochondria from diabetic vs. control rats. Kinetic studies in mitochondria from diabetic rats showed a reduction in maximum velocity and almost unchanged Michaelis-Menten constant of the CiC protein. Mitochondrial phospholipid amount was not significantly affected, whereas an increase in the cholesterol content and in the cholesterol/phospholipid ratio was observed. To thoroughly investigate the mechanism responsible for the reduced CiC activity in the diabetic state, molecular studies were performed. Ribonuclease protection assays and Western blotting analysis indicated that both hepatic CiC mRNA accumulation and protein level decreased similarly to the CiC activity. The reduced mRNA level and the lower content of the mitochondrial CiC protein, might account for the decline of CiC activity in diabetic animals. To discriminate between the role played by hyperglycemia from that of hypoinsulinemia in the reduction of CiC activity and expression, studies were conducted administrating phlorizin or insulin to streptozotocin-diabetic rats. Our data indicated that both insulin and glucose affect CiC activity and expression in diabetic rats, although they act at different regulatory steps.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Citric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Phlorhizin,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/citrate-binding transport protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1945-7170
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
151
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1551-9
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:20203153-Analysis of Variance,
pubmed-meshheading:20203153-Animals,
pubmed-meshheading:20203153-Blood Glucose,
pubmed-meshheading:20203153-Blotting, Western,
pubmed-meshheading:20203153-Body Weight,
pubmed-meshheading:20203153-Carrier Proteins,
pubmed-meshheading:20203153-Cholesterol,
pubmed-meshheading:20203153-Citric Acid,
pubmed-meshheading:20203153-Diabetes Mellitus, Experimental,
pubmed-meshheading:20203153-Insulin,
pubmed-meshheading:20203153-Liver,
pubmed-meshheading:20203153-Male,
pubmed-meshheading:20203153-Mitochondria, Liver,
pubmed-meshheading:20203153-Mitochondrial Membranes,
pubmed-meshheading:20203153-Organ Size,
pubmed-meshheading:20203153-Phlorhizin,
pubmed-meshheading:20203153-Phospholipids,
pubmed-meshheading:20203153-RNA, Messenger,
pubmed-meshheading:20203153-Rats,
pubmed-meshheading:20203153-Rats, Wistar
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| pubmed:year |
2010
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| pubmed:articleTitle |
Reduced activity and expression of mitochondrial citrate carrier in streptozotocin-induced diabetic rats.
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| pubmed:affiliation |
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Laboratorio di Biochimica, Università del Salento, Via Provinciale Lecce-Monteroni, 73100 Lecce, Italy. gabriele.gnoni@unile.it
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| pubmed:publicationType |
Journal Article
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