Linked Life Data

2020198

Source:http://linkedlifedata.com/resource/pubmed/id/2020198

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-5-24
pubmed:abstractText
Point mutations involving codons 12, 13, and 61 of the N-ras gene are found in patients with acute myeloid leukemia (AML). We have developed a sensitive assay for the analysis of these mutations which we have called allele-specific enrichment. In this protocol the polymerase chain reaction (PCR) amplifies DNA with primers that introduce new restriction sites into the normal N-ras allele only. Digestion with the appropriate enzyme cleaves normal, but not mutant, alleles and this digested product provides a mutant allele-enriched template for a second round of amplification. The second PCR product is digested, Southern blotted and analyzed by allele-specific oligonucleotide (ASO) hybridization. This protocol is more sensitive than ASO hybridization alone and has revealed a minor clone in the DNA of a patient with AML. The method may be useful for the detection of minimal residual disease in a subset of patients in remission.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:geneSymbol
N-ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
160-1
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Allele-specific enrichment: a method for the detection of low level N-ras gene mutations in acute myeloid leukemia.
pubmed:affiliation
Kanematsu Laboratories, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't