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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2010-4-26
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pubmed:abstractText |
Dopamine inhibited phosphate transport in isolated renal brush border membrane vesicles and in cultured renal proximal tubule cells from wild-type but not from NHERF-1 null mice. Co-immunoprecipitation experiments established that NHERF-1 associated with D1-like receptors. In wild-type mice, dopamine stimulated cAMP accumulation and protein kinase C (PKC) activity in renal proximal tubule cells, an effect that was abolished by SCH-23390, a D1-like receptor antagonist. In NHERF-1 null kidney tissue; however, dopamine failed to stimulate either cAMP accumulation or PKC activity. Infection of proximal tubule cells from NHERF-1 null mice with adenovirus-green fluorescent protein-NHERF-1 restored the ability of dopamine to stimulate cAMP and PKC. Finally, in (32)P-labeled wild-type proximal tubule cells and in opossum kidney cells, dopamine increased NHERF-1 phosphorylation at serine 77 of the PDZ I domain of NHERF-1, a site previously shown to attenuate binding of cellular targets including the Npt2a (sodium-dependent phosphate transporter 2a). Together, these studies establish that NHERF-1 plays a key role in dopamine signaling and is also a downstream target of D1-like receptors in the mouse kidney. These studies suggest a novel role for the PDZ adapter protein NHERF-1 in coordinating dopamine signals that inhibit renal phosphate transport.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-11997325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12075354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12169661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12192091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12193606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12386143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12586353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-12917102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-14706863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-14976410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-15037630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-15070904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-15547113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-15890704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-16460281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-16705152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-17369255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-17580307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-17895247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-17975671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-18202147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-18615257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-18987113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-9560162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20200151-9621280
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D1,
http://linkedlifedata.com/resource/pubmed/chemical/Slc34a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Phosphate Cotransporter...,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1083-351X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
285
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13454-60
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pubmed:dateRevised |
2011-7-28
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pubmed:meshHeading |
pubmed-meshheading:20200151-Adenoviridae,
pubmed-meshheading:20200151-Animals,
pubmed-meshheading:20200151-Dopamine,
pubmed-meshheading:20200151-Dopamine Agents,
pubmed-meshheading:20200151-Ion Transport,
pubmed-meshheading:20200151-Kidney Tubules, Proximal,
pubmed-meshheading:20200151-Mice,
pubmed-meshheading:20200151-Mice, Knockout,
pubmed-meshheading:20200151-Opossums,
pubmed-meshheading:20200151-Phosphates,
pubmed-meshheading:20200151-Phosphoproteins,
pubmed-meshheading:20200151-Phosphorylation,
pubmed-meshheading:20200151-Protein Kinase C,
pubmed-meshheading:20200151-Receptors, Dopamine D1,
pubmed-meshheading:20200151-Signal Transduction,
pubmed-meshheading:20200151-Sodium,
pubmed-meshheading:20200151-Sodium-Hydrogen Antiporter,
pubmed-meshheading:20200151-Sodium-Phosphate Cotransporter Proteins, Type IIa
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pubmed:year |
2010
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pubmed:articleTitle |
Sodium-hydrogen exchanger regulatory factor 1 (NHERF-1) transduces signals that mediate dopamine inhibition of sodium-phosphate co-transport in mouse kidney.
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pubmed:affiliation |
Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. eweinman1440@yahoo.com
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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