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pubmed-article:20196153pubmed:abstractTextThe apoferritin protein and apoferritin-Tb(3+) complex were demonstrated to form oligomeric and polymeric self-assemblies in neutral aqueous solutions, based on characterization by using luminescence and UV/Vis spectroscopy, dynamic light scattering, and transmission electron microscopy. Addition of a 20-mer or higher poly(arginine) to the solution resulted in coprecipitation through nanoscale interactions, while biological proteins and other poly(amino acids) rarely yielded precipitates under the conditions employed. The apoferritin-Tb(3+) complex assembly exhibited a particularly long-lived green luminescence in aqueous solution, and its poly(arginine)-selective precipitation behavior was followed by monitoring the changes in luminescence. The poly(arginine)-tagged albumin precipitated selectively and quantitatively, so that the apoferritin-Tb(3+) complex can function as a new luminescent biotool for the sensing of poly(arginine) and its protein conjugates.lld:pubmed
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pubmed-article:20196153pubmed:articleTitlePoly(arginine)-selective coprecipitation properties of self-assembling apoferritin and Its Tb(3+) complex: a new luminescent biotool for sensing of poly(arginine) and its protein conjugates.lld:pubmed
pubmed-article:20196153pubmed:affiliationDepartment of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. tsukube@sci.osaka-cu.ac.jplld:pubmed
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